宫颈癌中PAI-1、TGF-β1的表达及临床意义

Expression and signification of the type-1 inhibitor of plasminogen activator(PAI-1),transforming growth factorβ1(TGF-β1) in cervical carcinoma

目的:探讨纤溶酶原激活物抑制物-1(PAI-1)、转化生长因子-β1(TGF-β1)在宫颈癌中的表达及其在宫颈癌发生、发展中的作用。方法:采用Envision二步免疫组织化学方法检测宫颈癌石蜡包埋组织(63例,分为原位癌组、高分化组、中低分化组)与正常宫颈石蜡包埋组织(15例,对照组)中PAI-1、TGF-β1的表达。结果:(1)PAI-1在对照组、宫颈原位癌组、高分化组、中低分化组中的阳性表达率分别为0(0/15)、75.00%(6/8)、51.85%(14/27)、82.14%(23/28);对照组0(0/15)与宫颈癌组68.25%(43/63)间PAI-1蛋白阳性表达率差异有统计学意义(P<0.01);宫颈癌各组间PAI-1蛋白阳性表达率差异有统计学意义(P<0.01),PAI-1与病理分级有相关性。(2)TGF-β1在对照组、宫颈原位癌组、高分化组、中低分化组的细胞胞浆中的阳性表达率分别为13.33%(2/15)、25.00%(2/8)、48.15%(13/27)、71.43%(20/28);对照组13.33%(2/15)与宫颈癌组55.56%(35/63)间TGF-β1蛋白阳性表达率差异有统计学意义(P<0.01);TGF-β1在各组间细胞胞浆中阳性表达率差异有统计学意义(P<0.01),与病理分级有关;而在各组细胞外基质中的阳性表达率分别为13.33%(2/15)、37.50%(3/8)、22.22%(6/27)、42.86%(12/28);对照组与宫颈癌组33.33%(21/63)间基质中TGF-β1表达率差异无统计学意义(P>0.05)。(3)在宫颈癌中TGF-β1对PAI-1的表达有正协同作用(r=0.282,P<0.05);其表达率与年龄、民族无关。结论:在宫颈癌组织中PAI-1、TGF-β1的异常表达提示与肿瘤的发生、发展密切相关,并促进肿瘤的浸润与转移,联合检测PAI-1、TGF-β1有可能成为预测子宫颈癌发展及预后的重要指标。

Objective： To study the expression of the type 1 inhibitor of plasminogen activator （PAI-1）, transforming growth factorβ1 （TGF β1 ） in cervical carcinoma and analyze the influence of cervical carcinoma. Method. PAI-1, TGF-β1 in cervical carcinoma were determined by EnVision immunohistological method and compared between tumor specimens of cervical carcinoma （n = 63, including carcinoma in situs of cervix, high differentiation, middle low differentiation） and normal cervix tissue （control n =15）. Result： （1） The positive frequency of PAI-1 were 0（0/15）, 75.00%（6/8）, 51.85%（14/27）, 82. 14%（23/ 28） in normal group, carcinoma in situs of cervix, high differentiation, middle-low differentiation; the positive frequency of PAI-1 in normal group （0） was lower than in cervical carcinoma （68.25%） signifi cantly（P〈0.01）; each group was significant （P 〈0.01）, being associated with path ological grading. （2） The positive frequency of TGF-β1 were 13.33%（2/15）, 25.00%（2/8）, 48. 15% （13/27）, 71.43%（20/28） in normal group, carcinoma in situs of cervix？ high differentiation, middle low differentiation; the positive frequency of TGFβ1 in normal group （13. 33%） was lower than in cervical carcinoma （55.56%） significantly （P〈0.01）; each group was significant （P〈0.05）, being associated with pathological grading; but the positive frequency of matrix in each group was not significant （P〉0.05）. （3） Expression of TGFβ1 cooperated with expression of PAI-1 in cervical carcinoma （r = 0. 282, P〈0.05） ; but expression of PAI-1, TGF-β1 were no relation of age, nation （P〉0. 05）. Conclusion： Abnormal expression of PAI-1, TGF-β1 in cervical carcinoma indicate what were relation of occurring and development, contributing to the invasion of cervical caicinoma, it was useful to forecast development of cervical carcinoma by combining to examine.