基质金属蛋白酶及其抑制剂与咽喉部黑色素瘤浸润和转移的分子机制

Expressions of Matrix Metalloproteinase 2(MMP-2) and Tissue Inhibitor of Metalloproteinase 2(TIMP-2) and their Correlation with molecular-mechanism of invasion and metastasis in laryngopharyngeal malignant melanoma

目的:探讨基质金属蛋白酶(MM P-2)及组织基质金属蛋白酶抑制剂(T IM P-2)在咽喉部恶性黑色素瘤中的表达及其在肿瘤浸润转移中的作用。方法:将28例咽喉部恶性黑色素瘤样本按照微血管密度和K i-67标记指数分别分为高度恶性和低度恶性2组,并进行MM P-2及T IM P-2免疫组化染色。结果:(1)微血管计数>36.5的肿瘤组中MM P-2阳性表达率高于微血管计数<36.5的肿瘤组;而T IM P-2阳性表达率低于微血管计数<36.5的肿瘤组,两组差异有显著性(P<0.05);(2)K i-67标记指数>22.5的肿瘤组中MM P-2阳性表达率高于K i-67标记指数<22.5的肿瘤组;而T IM P-2阳性表达率低于K i-67标记指数<22.5的肿瘤组,两组差异有显著性(P<0.05)。结论:肿瘤浸润转移相关蛋白在肿瘤的浸润转移过程中作用各不相同,MM P-2促进了肿瘤的浸润转移,而T IM P-2抑制肿瘤的浸润转移。

Objective .. To study the expressions of Matrix Metalloproteinase 2 and Tissue Inhibitor of Metalloproteinase 2 and their Correlation with molecular-mechanism of invasion and metastasis in malignant melanoma. Methods：28 cases of malignant melanoma were divided into high and low grade groups according to the MVD and Ki-67 ,MMP-2 and TIMP-2 were detected by immunohistoehemieal staining. Results：Expressions of MMP-2 in high grade malignant melanoma group were higher than those in low grade group. Expressions of TIMP-2 in low grade malignant melanoma group were higher than those in high grade group. There were statistical significance of MMP-2 and TIMP-2 expressions between high and low grade malignantmelanoma （P^01 05）. Conclusion..Invasion and metastasis related proteins exerted different functions in the invasion and metastasis of tumors. MMP-2 could accelerate invasion and metastasis in tumors, but TIMP-2 inhibit the process.