潘托拉唑对大鼠胃黏膜保护作用的研究

The Protective Effect of Pantoprazole on the Gastric Mucosa in Rats

目的:探讨潘托拉唑对大鼠胃黏膜保护作用。方法:在乙醇诱导大鼠胃黏膜损伤前,预先给予潘托拉唑、L-硝基-精氨酸甲酯(L-NAM E)静脉注射。测定胃黏膜血流量(GM BF)、胃液pH和胃黏膜NO2-/NO3-含量,并观察了胃黏膜损伤指数(U lcer index,U I)、溃疡坏死组织和中性粒细胞浸润严重程度的变化。结果:与模型损伤组比,潘托拉唑组大鼠U I明显降低(P<0.01),溃疡坏死组织和中性粒细胞浸润程度明显减轻(P<0.01)。预先用L-NAM E处理后,潘托拉唑保护胃黏膜损伤作用明显减弱。静脉注射潘托拉唑,可增加胃黏膜血流和胃黏膜NO2-/NO3-,L-NAM E可逆转这种作用,但对潘托拉唑抑制酸分泌作用无明显影响。结论:潘托拉唑对大鼠胃黏膜具有重要的保护作用,一氧化氮(N itricox ide,NO)介导了这种作用。

Objective ：To observe the protective effect of pantoprazole on the gastric mucosa in rats. Methods ：Before gastric mucosal lesion was established, in rats by pure alcohol ,pantoprazole and Nω-nitro-L-arginine methyl ester（L- NAME）were injected intravenously. Gastric mucosal blood flow （GMBF） ,pH of gastric juice,and gastric mucosal NO2^-/ NO3^- were measured ,and the changes of ulcer index and the severity of tissues necrosis and neutrophils infiltration were observed respectively. Results ：Ulcer index of the pantoprazole group was much lower than that of control group （P〈 0.01） ,and the degree of tissues necrosis and neutrophils infiltration were much milder than those of the control group （P〈0. 01）. The protective effect of pantoprazole was significantly decreased by prior administration of L-NAME. Pantoprazole administered intravenously obviously increased GMBF and gastric mucosal NO2^-/NO3^- ,which was prevented by pretreatment with L-NAME,but the antisecretory effect of pantoprazole was not affected by L-NAME. Conclusion：Pantoprazole played an important protective role in the gastric mucosa in rats,in which nitric oxide （NO）was involved .