可见光对原代培养人视网膜色素上皮细胞的光化学损伤

VISIBLELIGHTDAMAGEINTHEPRIMARYCULTUREDHUMANRETINALPIGMENTEPITHELIALCELLS

目的：探讨视网膜光损伤的发生机制。方法：利用２４００Ｌｘ白色荧光灯建立原代培养的健康成年人视网膜色素上皮（ｒｅｔｉｎａｌｐｉｇｍｅｎｔｅｐｉｔｈｅｌｉｕｍ，ＲＰＥ）细胞光化学损伤模型，进行透射电镜观察和超氧化物歧化酶（ｓｕｐｅｒｏｘｉｄｅｄｉｓｍｕｔａｓｅ，ＳＯＤ）检测。结果：光照后线粒体肿胀，核膜结构模糊不清，部分损伤严重的细胞细胞器减少或消失，胞质空化，并见髓鞘样变性等改变；光照组细胞内ＳＯＤ含量为对照组的４１％（Ｐ＜０．０５）。结论：本实验条件下，光照使ＲＰＥ结构损伤变性，细胞内ＳＯＤ含量减少，提示与自由基产生和不能清除以及细胞内膜结构脂质过氧化反应有关。

PURPOSE:Insearchofthemechanismforphoticretinalinjury.METHODS:Avisi-blelightdamagemodelwasestablishedintheprimaryculturedhealthy,adulthumanRPEcelsbyusingintensefluorescencelight(2400Lx).RESULTS:Electronmicroscopyrevealedswelingofthemitochondriaandobscurityofnuclearmembranousstructureinthelightdamagedcels.Thede-creaseordissolutionoforganele,vacuolizationofcytoplasmandmyelinicdegenerationwerefoundinsomeseverelydamagedcels.ThelevelofintracelularSODwasdecreasedto41%ofthatofthecontrols(P<0.05).CONCLUSION:ThestructureoftheRPEwasdamagedbythelightradiationandthelevelofintracelularSODwasdecreased.Thesesuggestedthelightdamagemightbeassoci-atedwiththeproductionoffreeradicalsandthelipidperioxidereactioninmembranousstructureofcel.