红参麦冬复方注射液对烫伤大鼠心肌损害的影响及机制

Effect of shenmai injection on myocardial injury of scalded rats and its mechanism

目的:观察红参麦冬复方注射液对烫伤大鼠早期心肌损害的影响并探讨其机制。方法:实验于2005-07/12在遂宁市人民医院药剂科进行。选择健康Wistar大鼠45只,单纯随机分为正常对照组5只、烫伤对照组20只和参麦组20只,后2组动物背部92℃水浴18s,造成30%TBSAⅢ度烫伤模型。参麦组烫伤后立即腹腔注射10mL红参麦冬复方注射液1支(商品名参麦注射液,杭州正大青春宝药业有限公司产品,批号001015,10mL/支,含红参、麦冬各1.0g),烫伤对照组注射等量生理盐水。观察大鼠烫伤后6,12,24,48h后心肌组织病理变化,检测心肌组织丙二醛浓度、超氧化物歧化酶活性及血清肿瘤坏死因子α水平(ELISA法)、乳酸脱氢酶(分光光度法)、肌酸激酶(分光光度法)水平,免疫组化法检测心肌热休克蛋白70蛋白表达变化。正常对照大鼠不经任何处理,过量麻醉后取材检测作支持对照。结果:45只大鼠全部进入结果分析。①烫伤对照组心肌细胞出现了变性坏死,参麦组在各时相点心肌病理变化均较烫伤对照组轻。②心肌组织丙二醛浓度:烫伤对照组在烫伤后各时相点均高于正常对照组,参麦组大鼠烫伤后6,12,24,48h丙二醛浓度只有烫伤对照组的57.7%,47.1%,49.3%和45.4%。③心肌组织超氧化物歧化酶活性:烫伤对照组大鼠烫伤后各时相点均低于正常对照组(P<0.01),参麦组则高于同时相点烫伤对照组(P<0.01)。④心肌组织热休克蛋白70蛋白表达:参麦组在烫伤后6,12,24,48h表达均高于烫伤对照组(0.445±0.041,0.436±0.034;0.847±0.078,0.608±0.055;0.997±0.91,0.818±0.045;1.147±0.148,0.769±0.045,P<0.01)。⑤血清血清肿瘤坏死因子α水平:参麦组在烫伤后6,12,24,48h均低于烫伤对照组[(3.14±0.31),(3.62±0.33)μg/L;(4.25±0.48),(5.67±0.51)μg/L;(4.57±0.53),(6.16±0.55)μg/L;(3.24±0.29),(4.47±0.45)μg/L;P<0.01]。⑥参麦组血清乳酸脱氢酶、肌酸激酶活性显著低于烫伤对照组(P<0.01)。结论:红参麦冬复方注射液可减轻大鼠烫伤后早期心肌损害,可能与减轻大鼠烫伤后心肌组织脂质过氧化反应程度以及减轻炎性因子水平有关。

AIM： To observe the effect of shenmai injection （SMI） on the early myocardial injury of scalded rats, and investigate the mechanism. METHODS： This experiment was done in the Department of Pharmacy, Suining People＇s Hospital from July to December in 2005. Forty-five healthy Wistar rats were included and divideded randomly into normal control group （5 rats）, scald control group （20 rats） and SMI group （20 rats）. Except the normal control rats, other rats were scalded on backs at 30% TBSA Ⅲ degree by 18-s water bath at 92℃. After scalds, rats in SMI group were treated with intraperitoneal injection of 10 mL SMI （Commodity name was Shenmai Parenteral Solution, containing 1.0 g red ginseng and 1.0 g ophiopogon root, produced by Chiatai Qingchunbao Pharmaceutical Co.,Ltd, batch number： 001015, 10 mL per solution）, while the rats in scald control group received 10 mL saline. At 6, 12, 24 and 48 hours postscald, the pathological changes, the activity of superoxide dismutase （SOD） and the concentration of malondialdehyde （MDA） of myocardium were detected. While the levels of tumor necrosis factor （TNF）-α, lactate dehydrogenase （LDH） and creatine kinase （CK） of serum were assessed with ELISA method and spectrophotography separately. The expression of heat shock protein （HSP） 70 was detected by immunohistochemistry staining. There was no management in normal control group, and the normal mice were measured as support controls after overdose-induced anesthesia. RESULTS： Totally 45 rats were involved in the result analysis. ①The pathological changes in SMI group were milder than that in scald control group, which appeared the degeneration and necrosis of myocardial cells.② The MDA concentrations at different time points after scald were higher in scald control group than in normal control group, and the concentration in SMI group were 57.7%, 47.1%, 49.3% and 45.4% respectively of those in scald control group at 6, 12, 24 and 48 hours post-scald.③Compared with scald control group, the SOD activity increased in normal control group and SMI group at the different time points after scald （P 〈 0.01）. ④The HSP-70 expressions were higher in SMI group than in scald control group at 6, 12, 24 and 48 hours post-scald （0.445±0.041, 0.436±0.034; 0.847±0.078, 0.608±0.055; 0.997±0.91, 0.818±0.045; 1.147±0.148, 0.769±0.045, P 〈 0.01）. ⑤The TNF-α level was lower in SMI group than in scald control group at 6, 12, 24 and 48 hours post-scald [（3.14±0.31）, （3.62±0.33） μg/L; （4.25±0.48）, （5.67±0.51） μg/L; （4.57±0.53）, （6.16±0.55） μg/L; （3.24±0.29）, （4.47±0.45） μg/L; P 〈 0.01].⑤The levels of LDH and CK were both significantly lower in SMI group than in scald control group （P 〈 0.01）. CONCLUSION： SMI can relieve the early myocardial injury of scalded rats, which may has relationships with the decreases of lipid peroxidation and inflammatory factors.