刺五加叶皂苷单体Sc3对培养大鼠心肌细胞自发性搏动及动作电位的影响

Influence of Acanthopanax senticosides C 3 on spontaneous contractility and action potential of cultured rat myocardiocytes

目的：观察刺五加皂苷单体Ｓｃ３（ＡｃａｎｔｈｏｐａｎａｘＳｅｎｔｉｃｏｓｉｄｅＣ３）对培养的Ｗｉｓｔａｒ大鼠乳鼠心肌细胞自发性搏动及动作电位电参数的影响。方法：将５０～８００μｇ／ｍｌＳｃ３，分别加入到培养４～６ｄ后的Ｗｉｓｔａｒ大鼠乳鼠心肌细胞的培养液中，依次记录加药前、后自发性搏动及动作电位电参数的变化。结果：Ｓｃ３可剂量依赖性地抑制被培养的Ｗｉｓｔａｒ大鼠乳鼠心肌细胞的自发性搏动及动作电位的电参数，其作用与０．４μｇ／ｍｌ尼莫地平相似，而该作用被８０μｇ／ｍｌＣａ２＋所反转。

OBJECTIVE:To observe the influence of Sc 3(Acanthopanax senticoside C 3)on the spontaneous conrtractility and the electricalparameters of action potential of the cultured neonatal Wistar rat myocardicytes :50～800 μg/ml Sc 3 was added into the culture fluid of the neonatal Wistar rat myocardiocytes 4～6 days after culture,and then the changes of the spontaneous contractility and the electrical parameters of action potential recorded after and before administration.RESULTS:Sc 3 inhibited the spontaneous contractility and the electrical parameters of action potential of the cultured neonatal Wistar rat myocardiocytes dose dependently,which was similar to that of 0.4 μg/ml nimodipine,however,could be reversed by 80 μg/ml Ca 2+ .CONCLUSION:Sc 3 could block calcium channel.