Ge─132对烈酒精之氧化损伤的保护作用

ProtectiveefectsofGe┐132onoxidativedamageofstrongwine

目的：探讨β－羧乙基锗倍半氧化物（Ｇｅ－１３２）在体内清除自由基、抗氧化作用。方法：下列药物作用于烈酒造成氧应激态的大鼠，观察其肝线粒体超氧化物歧化酶活性和肝脏的病理变化，并与维生素Ｃ进行了比较。Ｗｉｓｔａｒ大鼠随机分为４组。正常对照（Ａ），烈酒（Ｂ），烈酒＋维生素Ｃ（Ｃ），烈酒＋Ｇｅ－１３２（Ｄ）。各组大鼠常规饲喂。每种药的给药方法如下：烈酒（含体积分数为５５％乙醇），５ｇ／（ｋｇｄ），从第８天开始ｉｇ共２周；维生素Ｃ２００ｍｇ／（ｋｇｄ），从第１天开始ｓｃ共３周；Ｇｅ－１３２２００ｍｇ／（ｋｇｄ），从第１天开始ｉｇ共３周。结果：肝线粒体铜锌超氧化物歧化酶活性，烈酒组与对照组比较，其活性下降（Ｐ＜０．０５），烈酒＋Ｇｅ－１３２和烈酒＋维生素Ｃ组均与烈酒组比较，其活性有上升趋势。肝脂肪变性在烈酒＋Ｇｅ－１３２组较烈酒组为轻，烈酒＋维生素Ｃ组病变不明显。结论：Ｇｅ－１３２在一定程度上保护肝线粒体铜锌超氧化物歧化酶活性，改善肝脂肪变性，但对肝脏病变的保护作用不如高浓度维生素Ｃ。

OBJECTIVE:Toexaminethepharmacologicalefectofβ-carboxyethylgermaniumsesquioxide(Ge-132)onscaveningfreeradicalandtheantioxidationinvivo.METHOD:Theefectsofthedrugsontheratsunderoxidativestressinducedbystrongwinewereinvestigatedbyobservingtheactivitiesofsuperoxidedismutase(SOD)inlivermitochondriaandpathologyofliverintherats.Wistarratswererandomlydividedintofourgroups(n=10):A:control;B:wine;C:wine+vitaminC;D:wine+Ge-132.Theratsinalgroupswerefedwithregularfood.Thedoseandrouteofadministrationofdrugsareasfolows:Strongwine(55%ethanol),5g/(kgd),igfrom8thdayfor2weeks.VitaminC200mg/(kgd)scfrom1stdayfor3weeks.Ge-132200mg/(kgd)igfrom1stdayfor3weeks.RESULTS:ActivityofCu,Zn-SODinlivermitochondriadecreasedinthestrongwinegroupcom-paredwiththecontrol(P<0.05),andtendedtoincreaseinthestrongwine+Ge-132groupandwine+vitaminCgroupcomparedwiththestrongwinegroup.Liversteatosiswaslessinthestrongwine+Ge-132groupthanthatinthestrongwinegroup,buttherewasnosignificantchangeinthestrongwine+vitaminCgroup.CONCLUSION:Ge-132couldprotectfromdecreasingtheactivityofCu,Zn-SODinlivermitochondriatoacertainextentandim-proveliversteatosisinstrongwinerat.TheefectsofGe-132inimprovingfatyliverwerenotbetterthanthoseofhighconcentrationvitaminC.