摘要
目的通过体外试验研究黄芪多糖(Astragalus mongholicus,ASP)对树突状细胞(dendrit- ic cells,DC)功能调节的机制,为进一步阐明黄芪多糖的免疫学活性提供依据。方法应用流式细胞仪检测技术、扫描电镜技术、酶联免疫吸附试验检测DC表型和功能的各种指标。结果本实验应用小鼠骨髓来源的DC,通过体外试验证明了黄芪多糖能够提高DC表面分子CD11c和MHCⅡ的表达,并且呈黄芪多糖浓度依赖性;空白组DC的吞噬功能很强,LPS组DC和黄芪多糖处理组DC吞噬功能都明显下降;黄芪多糖能够促进DC白细胞介素-12(IL-12)的表达;电镜观察DC的超微结构,可见黄芪多糖处理组DC突起增多,形态上更加成熟。结论本实验结果证实了黄芪多糖能促进小鼠骨髓来源的DC表型及功能的成熟。
Objective To explore the mechanism of immunomedulatory activity of ASP on routine bone marrow(BM)-derived dendritic cells(DC). Methods We used immunofluorescence staining and flow cytometry, scanning probe microscope, ELISA to determine the effect of ASP. Results ASP( 10, 50, 100, 250 μg/ml) could increase the co-expression of CD11c and MHC class Ⅱ molecules on DC surface. The ability of unstimulated DC to uptake FITC-dextran was higher than that of ASP- or LPS-treated DC. We analyzed the concentration of IL-12 secreted by DC using ELISA. ASP-treated DC secreted higher level of IL-12 than untreated DC. And ASP-or LPS-treated DC displayed a more matured morphology, with long protrusions, while untreated-DC displayed shorter protrusions than stimulated DC. Conclusion The results suggest that ASP can promote the phenotypic and functional maturation of DC.
出处
《中华微生物学和免疫学杂志》
CAS
CSCD
北大核心
2006年第7期637-640,共4页
Chinese Journal of Microbiology and Immunology
关键词
黄芪多糖
树突状细胞
成熟
Astragalus mongholicus polysaccharides
Dendritic cells
Matuaration