摘要
目的：建立血浆中华法林对映体浓度的立体选择性高效液相色谱法。方法：血浆标本经碱洗、酸化和乙醚提后，与碳苄氧基－Ｌ－脯氨酸进行柱前衍生化为差向体。以正己烷－乙酸乙酯－乙酸－甲醇为流动相，经普通硅胶柱分离对映体和在紫外λ３１３ｎｍ处检测。结果：对映体标准曲线范围０～４．０ｍｇ／Ｌ，最低检出限＜０．１ｍｇ／Ｌ，批内，批间平均ＲＳＤ均＜７％，平均回收率１００．２２％，临床上常用药物对本法没有干扰。结论：本法简便可靠，可用于临床华法林对映体的药动学和药效学研究。
OBJECTIVE:Astereoselectivehighperformanceliquidchromatography(HPLC)wasestablishedtodeterminewarfarinenantiomersinhumanplasma.METHOD:Theplasmawasextractedwithetherafteracid/basepurification.Aprecolumnderivatizationwasconductedbyusingcarbobenzyloxy-L-proline.Theenantiomerswereelutedwithhexane-aceticester-aceticacid-methanolanddetectedatλ313.RESULTS:Therangeofstandardcurveofeachenantiomerwas0～4.0mg/L,withthelowestdetectivelimitof0.10mg/Lorless.Theaveragerelativestandardofdeviation(RSD)ofwithinorbetween-assaywerelessthan7%andtheaveragerecoveryratewas100.22%.Commonlyco-administereddrugdidnotinterferetheassay.CONCLUSION:Themethodpermitscharac-terizationofthepharmacokineticsandpharmacodynamicsofwarfarinenantiomersinclinicalsetting.
出处
《中国药学杂志》
CAS
CSCD
北大核心
1996年第6期360-363,共4页
Chinese Pharmaceutical Journal
