Influences of enteral nutrition combined with probiotics on gut microflora and barrier function of rats with abdominal infection

瞄准：为了调查肠内的的、非肠道的营养和专业版的影响，生命学与腹的感染在肠的微生态学，上皮的紧密的连接，免疫者和老鼠的障碍功能上由内脏交付了。方法：与盲肠的结扎和穿孔方法建立的老鼠腹的感染模型，被划分成三个组：非肠道的营养(PN 组， n = 7 ) ， PN+enteral 营养(在组， n = 7 ) 并且在 + 职业人员生命学的 PN +( 职业人员生命学组， n = 7 ) 经由为五天的针空肠造口术和颈静脉。三个组的全部的营养的补充是 isonitrogenic 和 isocaloric。Probiotics 被空肠造口术交付 10 mL/d (1 x 10 (8 ) cfu/mL ) 。老鼠在第六天被打死。在盲肠的排汇物为厌氧的细菌的生长是有教养的并且与随机的放大多态的 DNA 与细菌的组 DNA 指纹侧面分析了。transmembrane 绑定蛋白质(occludin ) 和在在冒号和终端回肠的肠上皮的浆细胞的 IgA 水平被一个免疫组织化学方法测量。在冒号和小肠的肠的上皮的紧密的连接的超微结构被电子显微镜学观察。维纳腔血和肝，肺和 mes 伤寒淋巴结的 homogenated 织物是有教养的决定细菌的 translocations，并且在从门静脉的血的内毒素被检测。结果：(1 ) 内脏种类在的细菌的数量在组和职业人员，关於生命的组在 PN 是比那高级的组。DNA 侧面在在组和职业人员，关於生命的组类似于正常老鼠的。在职业人员生命学组的 DNA 侧面的数字在 PN 组并且在组是多于那的大部分。而且，在 PN 有奇怪的条纹组。( 2 ) occludin 和 IgA 在的表示小并且大肠在在组( 2.309 +/- 0.336 ， 15.440 +/- 2.383 )并且专业版关於生命的组( 2.938 +/- 0.515 ， 16.230 +/- 3.183 )作为与 PN 组相比被改进( 1.207 +/- 0.587 ， P 【 0.05 ， 11.189 +/- 2.108 ， P 【 0.01 )。在专业版的 occludin 的表达式关於生命的组(肠：2.93 +/- 0.515；盲肠：3.40 +/- 0.617 ) 比那高在在组(肠：2.309 +/- 0.336；盲肠：2.076 +/- 0.670；P 【 0.05 ) 。IgA 的表示，在特别在组(肠：15.440 +/- 2.383 ) 并且在组关於生命的专业版(大肠：12.516 +/- 1.542 ) 显著地作为与 PN 组相比增加了(肠：11.189 +/- 2.108；盲肠：10.160 +/- 1.643；P【0.01 ) 。肠的上皮的紧密的连接和职业人员的微绒毛关於生命的组是比在 PN 组的那些更未经触动的。( 3 )在内毒素的血，肝，肺和 mes 伤寒淋巴节点，和层次的细菌的 translocations 显著地在专业版被减少关於生命( 0.082 +/- 0.029 )并且在( 0.125 +/- 0.040 )作为与 PN 组相比组织( 0.403 +/- 0.181 ， P 【 0.05 )。结论：申请在与专业版生命学结合了能改进 transmembrane 绑定蛋白质(occludin ) 和 IgA 的表达式，改正肠内菌丛骚乱，维持内脏障碍函数和紧密的连接，并且减少出现毁坏细菌的易位。

AIM： To investigate the influences of enteral, parenteral nutrition and probiotics delivered by gut on intestinal microecology, epithelial tight junctions, immune and barrier function of rats with abdominal infection. METHODS： Rat abdominal infection models established with cecal ligation and perforation method, were divided into three groups： parenteral nutrition （PN group, n = 7）, PN＋enteral nutrition （EN group, n = 7） and PN ＋ EN ＋ probiotics （probiotics group, n = 7） via the needle jejunostomy and neck vein for five days. The total nutritional supplement of the three groups was isonitrogenic and isocaloric. Probiotics was delivered by jejunostomy 10 mL/d （1 x 10^8 cfu/mL）. The rats were killed on the sixth day. The feces in the cecum were cultured for anaerobic bacterial growth and analyzed with bacterial group DNA fingerprint profile with random amplified polymorphic DNA. The transmembrane binding proteins （occludin） and IgA level in plasma cells of intestine epithelium in colon and terminal ileum were measured by an immunohistochemistry method. The ultrastructure of intestinal epithelial tight junctions in colon and small intestine was observed by electronmicroscopy. Vena cava blood and the homogenated tissue of liver, lung and mesenteric lymph nodes were cultured to determine the bacterial translocations, and endotoxin in the blood from portal vein was detected. RESULTS： （1） The amount of bacteria of gut species in EN group and probiotic group was higher than that in PN group. The DNA-proflles in EN group and probiotic group were similar to that of normal rats. The number of DNAprofiles in probiotics group was much more than that in PN group and EN group. Moreover, there were strange stripes in PN group. （2） The expression of occludin and IgA in the small and large intestine in EN group （2.309 ± 0.336, 15.440 ± 2.383） and probiotic group （2.938 ± 0.515, 16.230 ± 3.183） was improved as compared with PN group （1.207 ± 0.587, P 〈 0.05, 11.189 ± 2.108, P 〈 0.01）. The expression of occludin in probiotic group （intestine： 2.93 ± 0.515; cecum： 3.40 ± 0.617） was higher than that in EN group （intestine： 2.309 ± 0.336; cecum： 2.076 ± 0.670; P 〈 0.05）. The expression of IgA, especially in EN group （intestine： 15.440 ± 2.383） and probiotic EN group （large intestine： 12.516 ± 1.542） significantly increased as compared with PN group （intestine： 11.189 ± 2.108; cecum： 10.160 ± 1.643; P 〈 0.01）. The intestinal epithelial tight junctions and microvilli of the probiotic group were more intact than those in the PN group. （3） The bacterial translocations in blood, liver, lung and mesenteric lymph nodes, and the levels of endotoxin were significantly reduced in probiotic （0.082 ± 0.029） and EN （0.125 ± 0.040） groups as compared with PN group （0.403 ± 0.181, P 〈 0.05）. CONCLUSION： Application of EN combined with probiotics could improve the expression of transmembrane binding proteins （occludin） and IgA, correct the intestinal flora disturbance, maintain gut barrier functions and tight junctions, and reduce the occurrence of gut bacterial translocation.