西北太平洋沉积物中细菌多样性的研究

Bacterial diversity in the sediments collected from the Northwest Pacific

利用提取且纯化的西北太平洋地区深海沉积物DNA为模板,利用细菌通用PCR引物扩增16S rDNA片段,构建其文库,建立阳性克隆子RFLP(Restriction Fragment Length Polymor-phism)酶切图谱.据酶切图谱选择部分克隆测序,并与数据库中的序列进行比对.结果表明,测序的27个序列分属于4个类群:变形细菌(Proteobacteria)、绿屈挠菌(Chloroflexi)、浮游霉菌(Planctomycetes)和酸杆菌(Acidobacteria);其中以变形细菌最多,占62.96%.

Diversity of bacteria was studied in deep-sea sediments from the Northwest Pacific by PCR, RFLP and sequence analysis of 16S rDNA and comparing with the published sequences in GenBank database. Based on the RFLP profile generated, 77 clones from the 16 s rDNA library were divided into 27 types. Phylogenetic analysis showed that the 27 independent clones fell into four groups： Proteobacteria （ 62.96 %）, Chloroflexi （ 14. 81%）, Planctomycetes （14. 81%） and Acidobacteria （7.41%）. Among all sequenced clones, 6 were related to the sulfate metabolism bacteria and the results also demonstrated that some bacteria in deep-sea sediments had relation to matter-energy circulation.