1,3-丙二醇氧化还原酶基因的克隆与序列分析

Gene Clone and Sequence Analysis of 1,3-Propanediol Oxidoreductase

以肺炎克雷伯氏菌的基因组DNA为模板,通过PCR扩增得到了目的基因dhaT,并将该基因克隆到pMD19-TSimple载体。基因序列分析表明,dhaT基因全长为1158bp,与GenBank上已发表基因序列的同源性达99%,氨基酸同源性为100%。

The gene of dhaT encoding 1,3-propanediol oxidoreductase was amplified by PCR and cloned into the vector of pMD19-T Simple. The sequence analysis showed that the gene of dhaT was 1158 bp that encoded a polypeptide of 387 amino acids. The deduced amino acid sequence homologies with Klebsiella pneumoniae ATCC25955 were 100 %.