Molt—4细胞内游离镁离子浓度的^（31）PNMR测定

THE DETERMINATION OF CYTOSOLIC FREE MAGNESIUM ION CONCENTRATION IN HUMAN LYPHOMA CELL LINE Molt-4 BY USING ￣(31)P NMR

通过测量人淋巴瘤白血病细胞ＭＯｌｔ—４的３１Ｐ谱中ＡＴＰ的α磷和β磷的化学位移差值，得出细胞内Ｍｇ２＋与ＡＴＰ结合的复合物ＭｇＡＴＰ和整个ＡＴＰ量的比值，计算得到Ｍｏｌｔ—４细胞内游离Ｍｇ２＋浓度为０．２５８ｍｍｏｌ／Ｌ．与其它分析方法相比．３１ＰＮＭＲ测定细胞内游离Ｍｇ２＋浓度具有对细胞样品无损伤的优点．

Based upon a measurement of chemical shift differences between the a phosphoryl and β phosphoryl resonances in the 31￣P spectrum of intracellular ATP in human lyphoma cell line Molt-4, the fraction of total ATP that is complexed to Mg￣2+ was estimated. Cytosolic free magnesium ion concentration in Molt- 4 cells was determined noninvasively to be 0.258 mmol/L.