摘要
用3H-TdR(氚标记的胸腺嘧啶脱氧核苷)掺入法结合DNA合成抑制剂处理,研究了纳秒级强脉冲电子束和Ar+离子束诱导的烟草种子的DNA损伤修复合成。受辐照种子在萌发的28~44h较正常种子有较多的3H-TdR的掺入,在萌发早期(60h内)出现了两个DNA合成峰,而正常种子仅有一个。受辐照种子内的第一个DNA合成峰受到DNA修复抑制剂咖啡因的强烈抑制,而第二个DNA合成峰受到DNA复制抑制剂羟基脲的强烈抑制。说明纳秒强脉冲电子束和Ar+离子束诱发了烟草种子DNA的损伤和修复。烟草种子的DNA修复合成开始于种子萌发的第28h,36h或40h达到高峰,较DNA复制高峰早12h或16h。本文还对DNA合成抑制剂的作用进行了讨论。
DNA repair synthesis of tobacco seeds induced by strong pulsed electron and Ar+ ion beams was studied with 3H-TdR incorporation method and DNA inhibitor treatment. It was found that there were much more incorporated 3H-TdR during 28~44 hours of germination in implanted seeds than that in normal seeds. and there were two DNA synthesis peaks during early stage of germination (Within 60 hours) in the implanted seeds, but only one peak in normal seeds. The first DNA synthesis peak of the irradiated seeds was strongly suppressed by caffeine which is an inhibitor of DNA repair synthesis. the second one was strongly suppressed by hydroxyurea which is an inhibitor of DNA replication. These results indicated that both of strong pulsed electron and Ar+ ion beams could induce the damadge and repair of DNA in tobacco seeds. DNA repair synthesis started after 28 hours of germination and reached peak at the 36 th or 40 th hour in irradiated seeds. The peak of DNA repair synthesis was 12 or 16 hours earlier than that of DNA replication.
出处
《辐射研究与辐射工艺学报》
CSCD
北大核心
1996年第4期222-225,共4页
Journal of Radiation Research and Radiation Processing
基金
甘肃省中青年科技基金
关键词
脉冲电子束
DNA修复合成
烟草种子
氩离子
Pulsed electron beam
Ar ̄+ ion beam
DNA repair synthesis
Tobacco seeds