大鼠再生肝细胞对四氯化碳的耐受

TOLERANCE OF REGENERATING RAT HEPATOCYTES AGAINST CARBON TETRACHLORlDE IN VITRO(ABSTRACT)

本工作采用肝部分切除(PH)后96h 大鼠的离体肝细胞,以细胞存活率、介质中 GPT、GOT 含量作为肝损伤指标,观察其抗四氯化碳损伤作用。在不加四氯化碳条件下,肝细胞温育3h 或5h 后,细胞存活率、漏入介质中GPT、GOT 活性在假手术组和 PH 组间无显著差异。但在有四氯化碳损伤条件下则不同,在温育3h 或5h 后,存活率在假手术组分别降到52.8±5.9%和43.5±5.8%,而 PH 组分别为69.2±5.8%和60.9±3.2%。漏出的 GPT和 GOT 活性在假手术组也明显高于 PH 组(P<0.01)。提示大鼠离体再生肝细胞有明显的抗四氯化碳损伤作用。

Hepatoprotective effect of regenerating rat hepatocytes on carbontetrachloride(CCl_4)toxicity was observed in vitro by determininigcell viability,leakage of GPT and GOT into medium.Hepatocyteswere isolated from regenerating rat liver 96h after partial hepatectomy (PH, 68%).There was no significant differenae of cell viability,GPTand GOT in the medium between PH and sham operated rats afterincubation for 3h and 5h without CCl_4.But,after CCl_4 injury,theresult was quite different that after 3h and 5h incubation,the cellviability decreased to 52.8±5.9% and 43.5±5.8% repectively in shamoperated rats,while only decreased to 69.2±5.8% and 60.9±3.2%repectively in PH rats.The leakage of GPT and GOT into the mediumin sham operated rats was remarkably higher than that in PH rats(P<0.01).The results indicate that regenerating rat hepatocytesshowed an obvious tolerance against CCl_4.