弓形虫PCR试剂盒对感染家兔血液和精液的检测

RESULTS OF TOXOPLASMA DETECTION IN RABBITS' BLOOD AND SEMEN BY PCR KITS.

目的:评价国内公司研制的弓形虫PCR检测试剂盒。方法:采用市售的国内生产的4种弓形虫PCR试剂盒检测弓形虫阳性兔的血液及精液标本。结果:温州伊利康TOX-PCR试剂盒、北京美迪科TOX-PCR试剂盒对家兔感染前后血液、精液标本均未出现阳性结果。上海复生TOX-PCR试剂盒检测家兔精液标本感染前阴性,感染后均能出现相应的结果,而检测血液标本杂带太多。洛阳华美TOX-PCR试剂盒检测家兔血液标本、精液感染前均为阴性,感染后血液标本阳性率76.53%,43份精液标本阳性率2.33%。结论:目前国内市售的TOX-PCR试剂盒可检测各种组织标本,但实际应用价值局限。

Objective： To evaluate the PCR kits which were developed and produced by domestic corporations. Methods： 4 types of kits from 4 corporations were enrolled into this research for toxoplasrna detection in blood or semen from rabbits and all detections were preformed as their directions indicated. Results： No positive result was detected in semen or blood samples from rabbits either before or after infection by TOX - PCR kits from Weizhou Yilikang Corp. and Beijing MDC Corp. ; All samples were negative before infection and turned to be positive after infection when they were detected by TOX - PCR kits from Shanghai Fusheng Corp., but there were too much non - specific bands; All samples were negative before infection, but 13 of 17 blood samples and 1 of 43 semen samples turned to be positive when kits from Luoyang Sino- Ame Corp. were used. Conclusion： As the directions indicated, these TOX - PCR kits marketed in China could be used for toxoplasma detection of samples from different tissues, but the practical application was very limited.