巴戟天对脐血CD34^+细胞体外扩增的影响

EFFECTS OF RADIX MORINDAE OFFICINALLS ON EX VIVO AMPLIFICATION OF CD34 CELL ISOLATED FROM CORD BLOOD.

目的:探讨巴戟天对脐血CD34+细胞体外扩增的影响。方法:用StemSpanTMSFEM无血清液体培养基加入不同浓度的巴戟天,进行液体扩增,计数总细胞数并检测CD34+细胞。收获扩增后的细胞用MethoCultTMH4435半固体培养基培养,计数CFU2E、BFU2E、CFU2GM集落。结果:巴戟天具有刺激CD34+细胞液体培养的作用。巴戟天的最佳浓度是50 mg/L,Rg1最佳浓度是5 mg/L,Rb1最佳浓度是5 mg/L。细胞总数分别扩增20.4、24.5、24.1倍,CD34+细胞数分别扩增4.22、5.19、4.12倍。在液体培养后仍可刺激扩增后脐血细胞集落形成,CFU2E集落形成能力分别比对照组高90.1%、70.8%、72.8%;BFU2E集落形成能力分别比对照组高53.4%、68.0%、67.0%;CFU2GM集落形成能力分别比对照组高67.0%、29.8%、39.4%。结论:巴戟天能促进造血干/祖细胞增殖,并且能诱导定向分化,具有类似生长因子和协同生长因子作用。

Objective： To investigate the effects of Radix Morindae Officinalls on ex vivo amplification of CD34 ＋ cell isolated from cord blood . Methods： The cells were amplified in Stem SPSnTM SFEM serum- free medium which was added to RMO of different concentrations for counting total cell numbers and monitoring CD34 ＋ cell. After amplification , the cells were cultured in semisolid medium of MethoCultTMH 4435 in order to count CFU, BFU-E and CFU- GM colonies.Results： RMO could stimulate CD34 ＋ cells in liquid culture system at the best concentrations of 50Lg/ml, 5mg/ml and 5mg/ml.The numbers of total cells in RMO groups were amplified by 20.4, 24.5 and 24.1 while the CD34 ＋ cell numbers were amplified by 4.22, 5.19 and 4.12. After cultured in liquid, CD34 ＋ cell could still stimulate the formation of colony of amplified cells.The abilities of the formation of colony of CFU - E were 90.1% , 70.8 % and 72.8 % higher than that in control group , the abilities of the formation of colony of BFU - E were 53.4% , 68.0% and 67.0% higher than that in control group, and the ability of the formation of colony of FU - GM were 67.0%, 29.8% and 39.4% higher than that in control group.Conclusion： RMO can stimulate proliferation and orienting differentiation of the hematopoietic stem cell. RMO has analogous and synergetic effects with the cytokines.