阴沟肠杆菌β内酰胺酶基因研究

Study on the genes of β-Lactamase in Enterobacter cloacae

目的了解临床分离的阴沟肠杆菌中β内酰胺酶基因存在状况。方法分离自临床44株阴沟肠杆菌,采用聚合酶链反应检测耐药基因(TEM、SHV、CTX-M-1群、OXA-1群、OXA-10群、DHA、ACT-1)。结果44株阴沟肠杆菌中TEM阳性24株(54.5%)、DHA阳性5株(11.4%)、ACT-1阳性35株(79.5%),其余基因均阴性。结论临床分离的阴沟肠杆菌TEM、DHA、ACT-1、基因携带率高。

Objective： To investigate the the genotyping of β - Lactamases （bla） in Enterobacter cloacae. Methods： 44 strains of Enterobacter cloacae were isolated from hospitalized patients, and the bla genes were detected by PCR. Results： The positive rates of genes of TEM, DHA and ACT - 1 were 54. 5%, 11.4% and 79. 5%. The other genes were not found in all 44 isolates tested. Conclusion ： There were very high positive percentages of TEM, DHA and ACT - 1 genes in Enterobacter cloacae isolated clinically.