内皮型一氧化氮合酶运输介导物在子痫前期患者脐血管中的表达

The study of endothelial nitric oxide synthase traffic inducer in the umbilical vessels of The Patients with Pre-eclampsia

目的研究内皮型一氧化氮合酶运输介导物(endothelial n itric oxide synthase traffic inducer,NOSTR IN)在子痫前期(pre-ec lampsia,PE)患者脐血管中的表达,探讨其在子痫前期发病中的作用。方法免疫组织化学法检测脐血管中NOSTR IN的表达,并通过高清晰度彩色病理图文分析系统对其定量分析;RT-PCR检测子痫前期患者脐血管组织中NOS-TR IN mRNA的表达。结果免疫组织化学显示正常妊娠和子痫前期患者脐血管中都有NOSTR IN的表达,但子痫前期患者脐血管内皮细胞胞浆染色为深棕色,而正常妊娠者脐血管内皮细胞胞浆染色为浅棕色;RT-PCR显示子痫前期患者脐血管组织中NOSTR IN mRNA的表达水平显著高于正常妊娠(P<0.01)。结论子痫前期患者脐血管组织中NOSTR IN表达增加,这可能是子痫前期发病机制的重要因素之一。

Objective： To investigate the expression of endothelial nitric oxide synthase traffic inducer （NOSTRIN） in the umbilical vessels of the patients with pre - eclampsia （PE） and discuss its role in the pathogenesis of PE. Methods ： Immunohistochemistry was used to detect the expression of NOSTRIN in umbilical vessels and HPIAS （ High Resolution Pathological Image Analysis System） - 1000 was used to determine the quantity of NOSTRIN ; The NOSTRIN mRNA in umbilical tissues was determined by RT -PCR analysis. Results; Immunohistochemistry showed that NOSTRIN expressed in endothelial cells of umbilical vessel in women with PE and normal group; RT - PCR showed that the expression level of NOSTRIN was significantly higher in women with PE than in the normal group （ P 〈0. 01 ）. Conclusion： It was concluded that the level of NOSTRIN expression increase in umbilical vessel of women with PE, indicating that this maybe involved in the pathogenesis of PE.