白癜风患者外周血单个核细胞IL-2 IL-10和IFN-γ的表达特点

Th1-type cytokine expression in peripheral blood mononuclear cells of patients with vitiligo

目的探讨白癜风患者中Th1/Th2型细胞因子表达的情况。方法采用细胞培养技术对白癜风患者和正常对照者的外周血单个核细胞(PBMC)在刀豆素A(ConA)作用下进行体外培养;用酶联免疫吸附试验(ELISA)检测培养上清中白细胞介素(IL)2、IL10,干扰素(IFN)γ含量。结果①白癜风患者PBMC产生IL2水平高于正常对照组(P<0.05),IL10水平低于正常对照组(P<0.01)、IFNγ水平高于正常对照组(P<0.05)。②患者和对照组PBMC加ConA刺激孔中IL2高于相应自然增殖孔(P<0.05,P<0.01)。结论白癜风患者Th1型细胞因子的表达增高,Th2型细胞因子的表达降低,可能在发病机制中起一定作用。ConA可刺激PBMC分泌细胞因子。

Objective To investigate the expression of Th1/Th2 cytokines in vitiligo patients. Methods Periferal blood mononuclear cells （PBMCs） from both v/filigo and normal controls were cultured with and without ConA. Culture supematants were assayed for IL-2, IL-10 and IFN-γ by enzyme-linked immunoadsorption assay （ELISA）. Resalts PBMCs from vitiligo patients produced more IL-2 （P〈0.05）,less IL-10 （P〈0.01） and more IFN-γ （P〈 0.05） than those from normal controls. The IL-2 produced in PBMCs from vitiligo patients and controls with ConA stimulating were significandy higher than their corresponding without ConA stimulating respeetivdy （P 〈 0.05, P 〈 0.01）.Conclusion The Th1-type cytokine expression of vitiligo patients in up-regulated, the Th2-type cytokine expression of vitiligo patients is down-regulated and which may play a role in the pathogenesis of vitiligo.