高效液相色谱法测定穿王消炎胶囊中两种成分含量

Determination of two ingredients in Chuanwang xiaoyan capsules by HPLC

目的：建立穿王消炎胶囊中穿心莲内酯和脱水穿心莲内酯的含量测定方法。方法：采用高效液相色谱法（HPLC），Hypersil ODS色谱柱；甲醇-水（55：45）为流动相；穿心莲内酯和脱水穿心莲内酯的检测波长分别为225nm和254nm;流速为1．0mL·min^-1。结果：穿心莲内酯在0．0772～0．6948μg、脱水穿心莲内酯在0．0502～0．4518μg范围内具有良好的线性关系，平均加样回收率分别为98．67％和99．03％，RSD分别为1．03％和1．48％。结论：本方法准确可靠，重现性好，可用于穿王消炎胶囊中穿心莲内酯和脱水穿心莲内酯的含量测定。

OBJECTIVE To establish a method for determining andrographolide and dehydroandrographlide in Chuanwang xiaoyan capsules by HPLC. METHODS The separation was performed on Hypersil ODS column, using methanol-water （55： 45） as mobile phase, the detection wavelengh of andrographolide and dehydroandrographlide was respectively at 225 nm and 254 nm; the flow rate was at 1.0 mL·min^-1. RESULTS The linear range of andrographolide and dehydroandrographlide was 0.0772-0.694 8μg and 0.0502-0.4518μg, the average recovery of andrographolide was 98. 67% （RSD=1.03%） and the