海南黎族哮喘患者血浆PAF及PAF-AH活性的观察

Observation on the activity of plasma PAF and PAF-AH in Li Nationality asthma patients in Hainan Province

目的探讨血小板活化因子(PAF)及乙酰水解酶(PAF-AH)等活性变化在黎族居民支气管哮喘发病机制中的意义。方法采用酶联免疫法等方法测定48例海南黎族支气管哮喘患者发作期和缓解期血浆PAF及PAF-AH的活性变化,同时观察总IgE和嗜酸细胞阳离子蛋白(ECP)水平。结果黎族哮喘发作期患者PAF、总IgE和ECP水平较正常组均明显增高,缓解期较发作期明显降低,均有显著意义(P<0.01),缓解期仍高于正常组,但无显著意义(P<0.05);发作期PAF-AH活性较正常组均明显降低,缓解组较发作组明显增高,均有显著意义(P<0.01),但缓解组仍低于正常组(P<0.05)。结论黎族哮喘患者急性发作期血浆PAF活性明显增高、PAF-AH活性明显降低,提示PAF和PAF-AH活性变化在黎族哮喘的发生机制中可能有一定作用,其临床意义有待于进一步研究。

Objective To investigate the significance of changes of plasma platelet - activating factor （PCF） and platelet - activating factor acetylhydrolase （PAF- AH） activity in Li nationality asthma patients. Methods ELISA and other methods were used to detect the activity of plasma platelet - activating factor acetylhydrolase, platelet - activating factor, serum total IgE and ECP in 48 Li nationality asthma patients. Results The levels of plasma PAF, serum total IgE and ECP at acute stage were significantly higher than that of the remission stage （ P 〈 0.01 ） and the levels of plasma PAF - AH was significantly lower than that of in remission stage （ P 〈 0.01 ）. In remission stage, The levels of PAF - AH was lower than that of normal subjects （ P 〈 0.05）. Conclusion The activity of PAF at acute stage was obviously chanced, while the activity of PAF - AH was markedly reduced, sugesting that the changes of activity of PAF and PAF - AH may ply certein role in the pathogenisis of asthma in LI Nationality and further study on its clinical significance is indicated.