慢性心衰气虚血瘀证大鼠模型的研究初探

Exploration of the Syndrome of Vital Energy Deficiency and Blood Stasis of CHF Rat Model

目的该研究旨在探索慢性心衰气虚血瘀证大鼠模型。方法选择Wistar雄性大鼠100只,开胸行左冠脉主干结扎术后缝合。将造模成功大鼠20只设为模型组,第2周开始减食喂养,每天让大鼠游泳1次;正常组为10只大鼠。4周后观察一般状态,测左室心功能和肾素活性(PRA)、血管紧张素Ⅱ(AngⅡ)、血栓素B2(TXB2)、纤溶酶原激活物抑制剂(PAI-1)及心脏结构的变化。结果动物一般状况观察:模型组倦怠,黄白色口唇,舌质黯、瘀斑,呼吸、心率加快。心功能和血液指标测定:模型组与正常组比较,P<0.01。心肌组织形态学观察:模型组心肌细胞核固缩,异染色质呈块团状,可见核仁,肌原纤维排列紊乱、断裂及缺失,各带难以分辨,肿胀,空泡化,闰盘间隙增宽,紧密连接及桥粘连接消失。结论慢性心衰气虚血瘀证模型大鼠符合中医气虚血瘀证。

Objective To explore the syndrome of vital energy deficiency and blood stasis of CHF rat model. Methods The Wistar male rats were deligated the main stem of left coronary artery. The succeeded twenty rats were cut the diet half from the second week, and swam exhausted every day, compared with normal group. After four weeks, the general condition, heart function, heart construction and NE, PRA, AngⅡ,TXB2, PAI-1 were observed. Results General condition： Model group seemed tired, pelage dimed, tongue ecchymosis, meanwhile breath and heart rate raised. Heart function： There was significant difference between model group and normal group （P〈0.01）. Heart construction：cardiac muscle cellkaryopyknosis, heterochromatin lumped, engorgement, vacuolization etc were found in model group. Conclusion The CHF rat model fit greatly for the syndrome of vital energy deficiency and blood stasis of TCM.