艾叶挥发油体内外抑菌作用的实验研究

Bacteriostatic Effect of Volatile Oil from Artemisia Argyi in Vitro and in Vivo

目的检测艾叶挥发油体内外抑菌作用。方法艾叶挥发油的最低抑菌浓度(MIC)检测采用琼脂二倍稀释法,并以红霉素为对照;其致死保护率检测是通过对感染小鼠进行试验性治疗来实现。结果艾叶挥发油对实验菌的MIC90值范围0.78～25μL/mL;对实验菌感染小鼠致死保护率40%～50%。结论艾叶挥发油体外对金黄色葡萄球菌、大肠杆菌、绿脓杆菌具有抑菌作用,其抑菌作用优于或等同于红霉素;体内对金黄色葡萄球菌、大肠杆菌、绿脓杆菌感染小鼠具有较好的保护作用。

Objective To study the bacteriostatic effect of the volatile oil from Artemisia argyi. Methods Minimal inhibitory concentration （MIC） was measured with double-fold didution method. Protective effect of the volatile oil from Artemisia argyi were determined by experimental treatment of infected mice in vivo. Results The MIC90 of 3 kind of pathogenic bacteria were 0.78-25μL/mL and protective rate for infected mice were 40%-50%. Conslusion The volatile oil from Artemisia argyi has better inhibiting effect on Staply lococcus aureus, Escherichiacoli and Pseudomona than Erythromycin. It has better protective effect for infected mice.