益妙保尔金米胶囊对结肠癌细胞株(CT-26)体内外生长的影响

Effect of Yimiaobao’erjinmi Capsule on Growth of Colon Cancer CT-26

目的:研究益妙保尔金米胶囊对小鼠结肠癌细胞株(CT-26)体内外生长的影响。方法:用改良MTT法测定益妙保尔金米胶囊对小鼠结肠癌细胞株(CR-26)体外生长的抑制率,计算半数抑制浓度IC50。将40只CT-26结肠癌皮下接种模型小鼠随机分成4组:对照组(等体积生理盐水)、香菇菌多糖片组(香菇菌多糖片,0.5mg/20g/日)、低剂量组(益妙保尔金米胶囊粉末,0.0834g/20g/日)、高剂量组(益妙保尔金米胶囊粉末,0.2501g/20g/日)。均采用接种5天后灌胃给药法,持续给药8天,停药24小时后,处死动物,剥离肿瘤,称重并计算脾指数及胸腺指数。结果:益妙保尔金米胶囊无明显的抑制小鼠结肠癌细胞株(CT-26)体外生长的活性,对小鼠结肠癌细胞株(CT-26)体内生长显示出了抑制作用,与生理盐水组比较,高剂量组的抑瘤率为30.26%,低剂量组的抑瘤率为25.74%。结论:益妙保尔金米胶囊对小鼠结肠癌细胞株CT-26体内生长有一定的抑制作用,且无明显的量效关系。

Objective:To investigate the effect of Yimiaobao’erjinmi Capsule on growth of colon cancer CT-26. Method:Use the improved method MTT to test Yimiaobao’erjinmi Capsule’s inhibitory rate on the mice colon carcinoma cell line CT-26, compute the IC50. The mice colon carcinoma cell line CT-26 was implanted subcutaneously in BALB/C mice. The mice were randomly divided into four groups: negative control group(saline solution), Xianggujunduotang tablet group (xianggu- junduotang tablet, 0.5mg.20g.d~ -1), higher group(Yimiaobao’erjinmi Capsule powder, 0.2501g.20g.d~ -1), lower group(Yimiaobao’erjinmi Capsule powder, 0.0834g.20g.d-1). All drugs were given to stomach after implanted the carcinoma cell line CT-26 5days. The mice were sacrificed after given drugs 8days, the tumors, the spleen and the thymus were processed for examination. Result: Compared with the negative control group, the growth of the implanted tumor was inhibited by Yimiaobao’erjinmi Capsule powder and the inhibitory rate of higher group was 30.26%, lower group was 25.07%. Conclusion: Yimiaobao’erjinmi Capsule can inhibit tumor growth, but without significant relationship between dose and curative effect.