中药方剂对肝纤维化大鼠肝脏ALR基因表达的影响

Effect of Chinese herbs on ALR mRNA expression in liver of the hepatic fibrosis rats

目的从分子水平探测中药治疗肝纤维化的作用机制。方法利用CCL4复合因素建立SD大鼠肝纤维化模型。实验动物分成正常对照组、模型组、中药治疗组3组,分别检测各组生化、肝纤维化指标和肝组织中ALR mRNA表达变化。结果ALT、AST、A/G、HA、PCⅢ、LN、PLD各指标:模型组均高于对照组(P<0.01),中药治疗组低于模型组(P<0.01),且HA、PCⅢ、LN、PLD的降低与病理组织反应肝纤维化的好转程度一致;肝组织中ALR mRNA的相对表达量:中药治疗组、模型组明显高于对照组(P<0.01),中药治疗组高于模型组(P<0.01)。结论本中药方剂可显著升高肝纤维化大鼠肝组织ALR mRNA表达。

Objective To study the molecular mechanism of treatment by Chinese herbs on the hepatic fibrosis of rats. Methods Hepatic fibrosis model of rats was established with CCL4. Then the rats were divided into control; model and Chinese herbs treated groups. The level of ALR mRNA expression, biochemical and hepatic fibrosis parameters were observed, from different groups. Results The concentrations of ALT, AST, A/G, HA, LN, PLD and PCⅢ in model group were higher than those in control group （P 〈 0.01 ）; those in Chinese herbs treated group were lower than in model group （P 〈 0,01）. The decrease of the levels of HA, LN, PLD and PCⅢ in Chinese herbs treated group were correlated with the improvement of hepatic fibrosis observed by pathology. The level of ALR mRNA expression both in Chinese herbs treated group and model group were significantly higher than that in control group （P〈0.01）. After treatment by Chinese herbs, the level of ALR mRNA expression in liver tissues was higher than that of control group （P 〈 0.01 ）. Conclusions Our Chinese herbs significantly enhanced the ALR mRNA expression in liver of hepatic fibrosis rats.