针刺心经干预急性心肌缺血大鼠心脏基因表达谱研究

Study on effects of acupuncture at the Heart meridian on gene expression pattern of heart in rats with acute myocardial ischemia

目的:从基因水平揭示针刺心经干预心肌缺血的作用机制。方法:采用结扎冠状动脉前降支法复制大鼠急性心肌梗死模型。比较肺经组与模型组、心经组与模型组的心脏基因表达谱变化。结果:与模型组比较,肺经组中大于2倍的差异表达基因(包括EST)有20个下调和14个上调,主要是免疫和炎性反应相关基因,细胞信号和传递蛋白相关基因等;心经组中有70个下调和20个上调,主要是离子通道和运输蛋白相关基因,细胞凋亡和应激反应蛋白相关基因,代谢相关基因,细胞信号和传递蛋白相关基因,DNA结合、转录和转录因子类基因,免疫和炎性反应相关基因等。结论:心经组、肺经组中大于2倍的差异表达基因的数目和类型有较大差异,提示针刺心经干预心肌缺血作用有相对特异性。

Objective To reveal the mechanism of acupuncture at the Heart meridian in treatment of myocardial ischemia from gene level. Methods A model of acute myocardial ischemia was made by ligation of left anterior descending branch of the coronary artery. Changes of gene expression pattern of the Lung meridian group, the Heart meridian group and the model group in the heart were compared. Results Differential expression genes and expression sequence tags （ESTs） were 14 with signal log ratio≥1 and 20 with signal log ratio ≤-1, mainly included immunological and inflammatory reaction relative genes, cell signal transduction and transferrin relative genes, and others, in the Lung meridian group vs the model group. Differential expression genes and ESTs were 20 with signal log ratio ≥1 and 70 with signal log ratio ≤-1, mainly included ion channel and transport protein relative genes, cell apoptosis and stress reaction protein relative genes, metabolism relative genes, cell signal transduction and transferrin relative genes, DNA＇s binding, transcription and transcripuonal factor relative genes, immunological and inflammatory reaction relative genes, ond others, in the Heart meridian group vs the model group. Conclusion In number and types of differential expression genes and ESTs with signal log ratio≥1 or ≤-1, the changes in the Heart meridian group vs the model group were obviously different from those in the Lung meridian group vs the model group, indicating that the Heart meridian has relative specificity in protecting against acute myocardial ischemia.