玉米蛋白粉中叶黄素提取及皂化工艺的研究

Study on lutein extraction and saponification technology from corn gluten meal

采用单因素试验和L9(34)正交试验,研究了玉米蛋白粉中叶黄素的提取和皂化工艺。在高效液相色谱仪上以外标法对叶黄素定量,分别考察溶剂种类、液固比、提取时间及温度、皂化时间及温度、KOH质量浓度对粗提液中叶黄素质量浓度的影响。研究结果表明:对试验选取的玉米蛋白粉,皂化使得叶黄素质量浓度从25.97μg/mL上升到35.77μg/mL,提高了37.74%;优化后的提取工艺参数为:液固比5 mL/g,时间0.5 h,温度25℃,叶黄素粗提率为67.94%;优化后的皂化工艺参数为:温度25℃、时间3 h、KOH质量浓度0.05 g/mL,叶黄素得率45.47%。提取叶黄素后的玉米蛋白粉可继续作为饲料使用。

Corn gluten meal （CGM） abundant in lutein is the outgrowth of cornstarch production by wet process. In this paper the process of lutein extraction and saponification from CGM were studied by both single-factor and orthogonal tests. Taking the HPLC as the determination method, the effects of vadous factors, including the sort of solvent, the ratio of liquid/solid （L/S）, extracting time and temperature, saponifying time and temperature, and mass concentration of ethanolic KOH, on the level of lutein content were investigated. The results indicated that saponification could remarkably raise the lutein level from 25.97 μg/mL to 35.77 μg/mL by 37.74% ; the optimal condition for extraction was that L/S： 5 mL/g, time： 0.5 hrs, temperature： 25℃, by which rough extraction ratio was 67.94% and the optimal condition for saponification was that time： 3 hrs, temperature： 25℃ with 0.05g/mL ethanolic KOH, by which the lutein yield was 45.47%.