摘要
目的采用RT-PCR方法来探讨桂枝汤干预Th1/Th2细胞漂移及机制。方法40只大鼠随机分为正常组(A组)、脾虚组(B组)、桂枝汤大剂量组(C组)、桂枝汤小剂量组(D组)等4组,每组10只;用规范的脾气虚证大鼠模型造模方法将B组、C组、D组大鼠造模,B组、C组、D组分别用蒸馏水、桂枝汤大剂量、桂枝汤小剂量灌胃,标本采集后用RT-PCR检测NFATmRNA、IL-4mRNA和IFN-γmRNA表达水平。结果脾虚组的NFATmRNA、IL-4mRNA表达上调;而IFN-γmRNA表达下调,经桂枝汤大小剂量组治疗后,NFATmRNA、IL-4mRNA表达下调,IFN-γmRNA表达上调。结论桂枝汤对Th1/Th2细胞漂移作用途径可能是通过下调NFATmRNA的表达,恢复了IFN-γmRNA正常转录水平,进而下调IL-4mRNA的表达,使脾虚大鼠Th1/Th2细胞达到一种新的平衡。
Objective To explore intervention of Guizhi decoction on Th1/Th2 cell shift in rats with asthenic spleen by RT-PCR. Methods 40 rats were randomly divided into four groups： normal group （A group）, asthenie spleen group （B group）, high-dosage group （C group） and low-dosage group （Ⅰ） group）. There were 10 rats in eaeh group, the asthenie, spleen model were made in B group, C group as well as D group. Rats in B group, C group, D group were fed with normal distilled water, high-dosage Guizhi decoction and lowdosage Guizhi decoction respectively ,samples were determined by PCR after being gathered. Results Expression of NFAT mRNA, IL-4 mRNA in B group were raised ,however, the expression of IFN-γ mRNA were decreased; after treatment, the expression of NFAT mRNA, IL-4mRNA in C group and D group were deereased and expression of IFN-γ mRNA were raised. Conclusion The possible way to intervene the Th1/Th2 eell shift is that Guizhi deeection adjust the expression of NFAT mRNA down, resume normal transcription of IFN-γ mRNA ,then adjust the expression of IL-4 mRNA down ,whieh reaeh a new balanee between Th, cell and Th2 cell.
出处
《江西医药》
CAS
2006年第7期454-457,共4页
Jiangxi Medical Journal
基金
江西中医学院中医科研项目资助项目
