摘要
将经超声处理的磷脂与待包埋的ＳＯＤ溶液混匀，在液氮中深度冷冻后于３５℃融化，经超声处理、离心即得ＳＯＤ脂质体。ＳＯＤ活性包埋率约为３０％。本法操作方便，所需设备简单，可避免直接与有机溶剂接触，适用于生物活性物质的包埋和大规模生产。
The SOD liposomes were prepared by freeze-thaw method. The lipids were mixed with SOD solution and frozen at -196℃ in liquid nitrogen and left to thaw at 35℃. The thawed milky solution was sonicated and centrifuged, and the SOD liposomes were obtained. The encapsulation efficiency of this process is about 30%. The method is simple and is superior to others for avoiding organic solvent inactivation to biological substance.It can be used to encapsulate most biological molecules and be easily scaled-up.
出处
《中国医药工业杂志》
CAS
CSCD
北大核心
1996年第8期346-349,共4页
Chinese Journal of Pharmaceuticals
关键词
脂质体
SOD
冻融
制备
liposome, SOD, freeze-thaw method, Preparation
