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固定化金霉素产生菌原生质体使林可霉素转化的研究 被引量：1

STUDIES ON REACTION OF IMMOBILIZED PROTOPLAST OF S.aureofacicns FOR INVERSION OF LINCOMYCIN
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摘要 用固定化金霉素产生菌（金霉素链霉菌，Ｓ．ａｕｒｅｏｆａｃｉｅｎｓ）的原生质体，在适当量的氯离子存在时，转化林可霉素生成抗菌活力比其本体高的产物。研究了反应时间、原生质体固定化浓度、填抖比、碳源、氮源、载体厚度、摇床转速和载体琼脂浓度等因素对转化反应的影响。经３２ｈ转化培养后，反应液抗菌活力最高可提高一倍以上。 It has been indicated that antibiotic activity of lincomycin reaction solution increased twofold in after 32h reaction with immobilized protoplast of S.aureofaciens.The effect of reaction time,concentration of protoplast in media,packing factor etc.on inversion reaction has been studied.
作者 徐京宁 米贯东 李凡超 唐孝宣
机构地区 华东理工大学生物化学工程研究所
出处 《中国医药工业杂志》 CAS CSCD 北大核心 1996年第10期440-442,共3页 Chinese Journal of Pharmaceuticals
关键词 林可霉素 金霉素 链霉菌 固定化 原生质体 lincomycin,S.aureofaciens,immobilized protoplast
分类号 TQ465.4 [化学工程—制药化工]
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  • 2Hansen PJ,Thatcher WW, Ealy AD. Methods for reducing effects of heat stress on pregnancy. In:VanHom HH,Wilcox CJ(eds),Large Dairy Herd Management. American Dairy Science Association, Champaign, IL. 1992, 116-125.
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  • 5Battaglia DF, Krasa HB,Padmanabhan Vet al. Endocrine alterations that underlie endotoxin-induced disruption of the follicular phase in ewes. Biol Reprod. 2000, 62:45-53.
  • 6Suzuki C,Yoshioka K, Iwamura Set al. Endotoxin induces delayed ovulation following endocrine aberration during the proestrous phase in Holstein heifers. Domestic Animal Endocrinology. 2001.20 (4): 267-278.
  • 7Leung ST, Cheng Z, Sheldrick EL et al. The effects of lipopolysaccharide and interleukins-1alpha, -2 and -6 on oxytocin receptor expression and prostaglandin production in bovine endometrium. Journal of Endocrinology. 2001, 168:497-508.
  • 8尹保民 赵玉奎 金庭辉.应用促孕灌注液治疗奶牛不孕症的体会.中国畜牧杂志,1999,1:47-47.
  • 9Guilbault L A, Thatcher W W, Drost Met al. Source of F series prostaglandins during the early postpartum period in cattle. Biol Reprod.1984,31:879-887.
  • 10Marc Drillich,Damaris Raab,Miriam Wittke et al. Treatment of chronic endometritis in dairy cows with an intrauterine application of enzymes:A field trial. Theriogenology. 2005, 63:1811-1823.

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中国医药工业杂志
1996年 第10期

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