胃癌及周围淋巴结中PTEN基因蛋白表达的研究

Expression of PTEN protein in the gastric carcinoma and peripheral lymph nodes

目的研究PTEN基因在胃癌原发灶及周围淋巴结组织中的表达,以探讨其癌基因在胃癌的发生、发展及转移过程中的作用。方法用免疫组织化学方法检测62例胃癌的原发灶、癌旁组织及周围淋巴结的PTEN表达情况,同时结合病人的病理资料进行分析。结果62例胃癌原发灶PTEN总阳性表达率:41·9%(26/62);癌旁组织为100·0%(62/62);癌转移淋巴结组织中PTEN总阳性表达率:31·4%(27/86);无癌转移淋巴结为92·1%(35/38)。胃癌原发灶PTEN阳性表达率明显低于癌旁组织,而明显高于淋巴结转移癌,差异均有统计学意义(χ2=8·41和χ2=6·75,P<0·01);淋巴结转移癌组织的PTEN阳性表达率低于无癌转移淋巴结,差异有统计学意义(χ2=8·34,P<0·01)。N1、N2站癌转移淋巴结中PTEN的阳性表达率分别为32·1%(18/53)、30·3%(10/33),两者比较差异无统计学意义(χ2=2·31,P>0·05)。原发灶PTEN蛋白阳性表达率与周围淋巴结转移呈负相关。结论抑癌基因PTEN的变化与胃癌的淋巴结转移存在相关关系。

Objective To investigate the expression of PTEN protein in human gastric cancer and its relative lymph nodes, so to explore its role in the development and metastasis of gastric cancer, Methods The expression of PTEN protein in 62 eases of gastric primary carcinoma, the matched adjacent normal gastric tissue and peripheral lymph nodes were detected by immunohistochemical S-P technique, which was analyzed with patients＇ pathological data. Results The expression rate of glEN in gastric cancerous focus（41.9% ,26/62） was lower than that in the matched adjacent normal gastric tissue （100.0% ,62/62） ,but was higher than that in the lymph node with metastasis of gastric career（31.4%, 27/86）, with statistic significanee（X^2 = 8.41 and X^2 = 6,75, respectly, P 〈 0.01）.The Expression rate of PTEN in peripheral lymph nodes with metastasis（31.4% ,27/86） was lower than that without metastasis（92,1% ,35/38）（X^2 = 8.34, P 〈 0.01） ,but there was no difference between in N1（32.1%, 18/53） and in N2（30.3% （10/33） with lymph nodes metastasis （X^2 = 2.31, P 〉 0.05）.There was negative correlation between the expression of PTEN and lymph node metastasis. Conclusions The tumor suppressor gene FrEN plays some roles in the lymph nodes metastasis of human gastric cancer,