HPLC法同时测定咳喘清滴丸中盐酸麻黄碱、盐酸伪麻黄碱、氢溴酸东莨菪碱的含量

Determination of the contents of ephedrine hydrochloride,pseudoephedrine hydrochloride and scopolamine hydrobromide in Kechuanqing dripping pills by HPLC

目的建立咳喘清滴丸中盐酸麻黄碱、盐酸伪麻黄碱、氢溴酸东莨菪碱的含量测定方法。方法采用船LC法，色谱柱为Eclipse XDB-C8（150mm×4．6m，5μm），流动相为甲醇-0．02mol·L^-1磷酸二氢钾溶液-0．03mol·L^-1磷酸溶液（体积比为5．0：47．5：47．5），流速为1．0mL·min^-1，检测波长为210nm。结果对照品盐酸麻黄碱、盐酸伪麻黄碱、氢溴酸东莨菪碱分别在21～210、20～200、5～50ng内与色谱峰面积呈良好的线性关系，其相关系数均为r=0．9999，平均加样回收率分别为100．84％、100．39％、97．86％，RSD分别为1．45％、1．43％、1．51％（n=5）。结论测定方法可作为咳喘清滴丸质量控制方法。

Objective To establish a HPLC method for simultaneous determination of ephedrine hydrochloride, pseudoephedrine hydrochloride and scopolamine hydrobromide in Kechuanqing （traditional chinese medicines） dripping pills. Methods The column of Eclipse XDB-C8（4.6 min× 150 mm, 5 μm） was used, the mobile phase was MeOH-0.02 mol·L^-1 KH2PO4-0.03 mol·L^-1 H3PO4 （ V ： V ： V = 5.0 ： 47.5 ： 47.5）, the detection wavelength was set at 210 nm and the flow rate was 1.0 ml·min^-1. Results The linear range of ephedrine hydrochloride was 21-210 ng, pseudoephedrine hydrochloride was 20-200 ng and scopolamine hydrobromide 5 - 50 ng respectively. The average recoveries of ephedrine hydrochloride, pseudoephedrine hydrochloride and scopolamine hydrobromide were 100.84 % with RSD 1.45 %, 100.39 % with RSD 1.43 %, 97.86 % with RSD 1.51% （ n = 5）. Conclusions The method is accurate, sensitive and suitable for the quality control of Kechuanqing dripping pills.