摘要
Aspergillus oryzae 3042 mycelium pellets were immobilized by crosslinking method with reagents of gelatin and formaldehyde. An orthogonal design table was used to determine the optimal immobilization conditions. The L-aminoacylase activity of immobilized mycelium pellets under optimal conditions was assayed. The results show that the L-aminoacylase activity was 1350 U·g^-1 and the activity retention rate was 83%. The properties of the immobilized preparation were studied. Compared with free pellets, the appropriate pH of reaction system changed from 7.0 to 8.0, temperature changed from 52℃ to 63℃, and the ranges of the optimum reaction conditions were all improved. The effect of Co^2＋ on immobilized mycelium pellets was investigated and the favorable concentration was determined. When the immobilized preparation was used for the resolution of D, L-alanine in a packed bed reactor with 10 mm in diameter, 200 mm in height, the operational stability was increased, and the half-life period was 53 d. Thermal stability analysis shows that the immobilized pellets were more stable than the free pellets.
曲霉属菌 oryzae 3042 个菌丝体小团被 crosslinking 方法与明胶和甲醛的试剂使不能调动。一张直角的设计桌子被用来决定最佳的固定条件。在最佳的条件下面的使不能调动的菌丝体小团的 L-aminoacylase 活动是 assayed。结果证明 L-aminoacylase 活动是 1 350 Uo g^(-1), 和活动保留率是 83% 。使不能调动的准备的性质被学习。与免费小团相比，反应系统的适当酸碱值从 7.0 ～ 8.0 变化了，从 52 deg C 改变到 63 deg C 的温度，和最佳反应的范围调节都被改进。使不能调动的菌丝体小团上的 Co^(2+) 的效果被调查，有利集中是坚定的。当使不能调动的准备被用于 D 的决定时，在有在直径的 10 公里的一个填充床反应堆的 L 丙氨酸，在高度的 200 公里，运作的稳定性被增加，并且半衰期时期是 53 d。Thermalstability 分析证明使不能调动的小团比免费小团更稳定。
