摘要
建立了棕榈油中生育酚和生育三烯酚的准确、快速的HPLC分析方法。选用Nucleosil硅胶柱正相分离,以正己烷-异丙醇为流动相,梯度洗脱,在紫外波长292nm下进行检测,获得较理想的分离效果。该方法线性范围为0.2275～1.3031mg/mL,平均加样回收率为100.17%,RSD=0.5905%。
An HPLC method has been established for the determination of α,β,γ,δ-tocopherol and α,β,γ,δ- tocotrienol in palm oil. The samples are analyzed by HPLC using a Nucleosil column （4.6×250 mm, 5μm） and UV detection at 292 nm. The mobile phase is a mixture of hexane and 0.5% isopropanol in hexane with gradient dilution. The linear correlation is good in the range of 0.2275-1.303mg/mL. The average recovery rate is 100.17%, RSD=0.59%
出处
《食品工业科技》
CAS
CSCD
北大核心
2006年第7期177-178,181,共3页
Science and Technology of Food Industry
