摘要
目的:研究白芨胶对体外培养的大鼠皮肤成纤维细胞VEGFmRNA表达的影响,探讨白芨胶促进伤口愈合的分子机制。方法:体外培养大鼠皮肤成纤维细胞,分别给予不同浓度的白芨胶进行干预,然后用RT-PCR技术分别检测各组细胞的VEGFmRNA表达,并进行定量分析。结果:白芨胶在不同浓度时连续干预至第3d,以0.5mg/ml浓度条件下,VEGFmRNA表达最强。结论:白芨胶对体外培养的大鼠皮肤成纤维细胞VEGFmRNA表达有明显促进作用,其作用强度随浓度而改变。
Objective：To study the effects of Bletilla hyacinthine gum（BGH） on the mRNA expression of VEGF in rat fibroblast in vitro and explore its possible molecular mechanisms in promoting the wound healing. Methods：The rat fibroblast cultured in vitro were stimulated with BGH according to the protocol. The expression of mRNA of VEGF in the cells in every group was examined by reverse-transcriptase ploymerase chain reaction （RT-PCR）. Results： When fibroblast stimulated with different concentrations of BGH for 3 days, the expression of VEGF mRNA peaked with 0.5mg/ml BGH on the 5th day. Conclusion： BGH could promote significantly the expression of VEGF mRNA in rat fibroblast in vitro. The levels of expression of VEGFmRNA were changed with different concentrations of BGH.
出处
《中国中医骨伤科杂志》
CAS
2006年第4期15-16,共2页
Chinese Journal of Traditional Medical Traumatology & Orthopedics
