PCR检测深部致病真菌感染的研究

Detection of deep-seated pathogenic fungi by polymerase chain reaction

目的探讨PCR在可疑深部真菌感染临床标本检测中的应用。方法对60份临床标本分别进行传统真菌培养以及真菌通用引物PCR和三重PCR检测。结果真菌培养阳性共19份,其中白念珠菌10份、烟曲霉2份、新生隐球菌2份、其他菌株5份(包括光滑念珠菌2份、热带念珠菌2份、克柔念珠菌1份)：真菌通用PCR检测共有21份阳性,经三重PCR检测共有15份阳性,分别为白念珠菌10份、烟曲霉3份、新生隐球菌2份。PCR方法与传统培养方法相比,检出率差异无统计学意义。结论PCR检测可疑深部真菌感染临床标本快速简便,稳定性好,敏感性和特异性均较理想,适合临床实验室常规使用。

Objective To study the clinical application of polymerase chain reaction （ PCR ） in the detection specimens from patients with suspected deep-seated pathogenic fungal infection. Methods Sixty clinical specimens were detected with pan-fungal PCR, triplex PCR and traditional fungal culture, respectively. Results Nineteen specimens were positive by culture, consisting of 10 strains of Candida albicans, 2 strains of Aspergillus fumigatus, 2 strains of Ctyptococcus neoformans and 5 other strains （ 2 strains of Candida glabrata, 2 strains of Candida tropicalis and 1 strain of Candida krusei） ; 21 were positive by pan-fungal PCR; and 15 were positive by triplex PCR, including 10 strains of Candida albicans, 3 strains of Aspergillus fumigatus and 2 strains of Ctyptococcus neoformans. There was no statistical significance in the detection rate of fungi between the PCR and culture methods. Conclusion The PCR procedure is rapid, convenient, and reliable for the detection of deep-seated pathogenic fungi with higher specificity and sensitivity, and may be routinely used in clinical laboratory.