期刊文献+

巢式PCR检测阿萨希丝孢酵母DNA 被引量:4

原文传递
导出
摘要 阿萨希丝孢酵母(Trichosporon asabii,T.asahii)是引起毛孢子菌病的主要病原菌。我们在2000年报道了国内首例播散性毛孢子菌病,其后在另一家医院也发现肺部T.asahii感染。该病不仅可有广泛的皮肤损害,还可引起肝、脾、肾、肺等内脏损害,死亡率高达80%以上。由于该病少见,临床医生很少将皮损活检标本及肝穿组织直接送做真菌培养,而多首选普通病理检查。在患者需要补充检查和回顾性诊断时,蜡块组织往往是惟一可以得到的标本。因此,如能通过蜡块组织扩增T.asahii DNA特异性片段来诊断T.asahii感染具有重要的临床意义。
出处 《中华皮肤科杂志》 CAS CSCD 北大核心 2006年第8期481-482,共2页 Chinese Journal of Dermatology
基金 国家自然科学基金(30570099)
  • 相关文献

参考文献4

二级参考文献3

  • 1中国普通微生物菌种保藏管理中心,菌种目录(第3版),1997年,85页
  • 2Cletus P. Kurtzman,Christie J. Robnett. Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences[J] 1998,Antonie van Leeuwenhoek(4):331~371
  • 3E. Guého,M. Th. Smith,G. S. Hoog,G. Billon-Grand,R. Christen,W. H. Batenburg Vegte. Contributions to a revision of the genusTrichosporon[J] 1992,Antonie van Leeuwenhoek(4):289~316

共引文献85

同被引文献27

  • 1李家文,丁娟,谭志建,刘志香.用拓扑异构酶Ⅱ基因区巢式PCR法鉴别常见皮肤癣菌[J].中华皮肤科杂志,2005,38(8):521-522. 被引量:1
  • 2Kano R, Matsuoka A, Kashima M,et al. Detection of Sporothrix schenckii chitin synthase 1 (CHS1) gene in biopsy specimens fi'om human patients with sporotrichosis. J Dermatol Sci, 2003, 33( 1 ): 73-74.
  • 3Kim J, Chae C. Optimized protocols for the detection of porcine circovirus 2 DNA from formalin-fixed paraffin-embedded tissues using nested polymerase chain reaction and comparison of nested PCR with in situ hybridization. J Virol Methods,2001, 92(2): 105-111.
  • 4Sato Y, Sugie R, Tsuchiya B, et al. Comparison of the DNA extraction methods for polymerase chain reaction amplification from formalin-fixed and paraffin-embedded tissues. Diagn Mol Pathol, 2001, 10(4): 265-271.
  • 5Greer CE, Peterson SL, Kiviat NB, et al. PCR amplification from paraffin-embedded tissues. Effects of fixative and fixation time. Am J Clin Pathol, 1991, 95(2): 117-124.
  • 6Mies C, Houldsworth J, Chaganti RS. Extraction of DNA from paraffin blocks for Southern blot analysis. Am J Surg Pathol, 1991, 15(2): 169-174.
  • 7Kosel S, Grasbon-Frodl EM, Arima K, et al. Inter-laboratory comparison of DNA preservation in archival paraffin-embedded human brain tissue from participating centres on four continents. Neurogenetics, 2001, 3(3): 163-170.
  • 8Zeng IL Li X, Chen X, el al. Identificalion of Peniillium marneffei in Paraffin-embedded lissue using nested PCR. Mycpathologia, 2009, 168( 1 ): 31-35.
  • 9Xie Z. Zhang J, Xi L, et al. A chronic chromoblastomycosis model by Fmsecnea monopbora in Wistar rat. Med Mcol 2010, 48(1 ):201-206.
  • 10Bialek R. Cirera AC, Hernnann T, et al. Nested PCR assays tbr detection of Blastomyees dermatitidis DNA in pmaffin-embedded ermine tissue. J Clin Microbiol, 2003, 41 ( 1 ): 205-208.

引证文献4

二级引证文献2

相关作者

内容加载中请稍等...

相关机构

内容加载中请稍等...

相关主题

内容加载中请稍等...

浏览历史

内容加载中请稍等...
;
使用帮助 返回顶部