华蟾素联合三氧化二砷抑制鸡胚尿囊膜血管生成的实验研究

Study the effect of antiangiogenesis of ansenic trioxide in combination with cinobufacin on chick embryo chorioallantoic membrane (CAM)

目的:观察和比较华蟾素注射液、三氧化二砷注射液单药以及联合用药后对鸡胚尿囊膜(CAM)血管生成的抑制作用。方法:100只七日龄鸡胚随机分为4组(每组25只):生理盐水(NS)对照组、华蟾素组、As2O3组和华蟾素联合As2O3组。CAM上植入甲基纤维素碟,加入各组药物,观察对血管生长的影响。结果:华蟾素、As2O3以及两药联合对鸡胚尿囊膜新生血管均有抑制作用,以两药联合后抑制血管的作用最强。结论:华蟾素、As2O3以及两药联合均具有抗血管生成作用,两药联用后且具有协同作用。

Objective：To observe and compare the effects of antiangiogenesis in CAM between As20s and cinobufacin. Methods： One hundred living chicken embryo cultured for 7 days were divided into four groups （ twenty-five pieces each group ） ： As2O3 in combination with cinobufacin, cinobufacin, As2O3, NS control group, with methyl cellulose discs as carriers, which were incoubated together, observed their effects of angiogenesis in CAM. Results：The inhibitive effects of angiogenesis in CAM is significant among groups of As2O3, cinobufacin and combining group, which was the most strong for the combination drug group. Conclusion：It is a positive cooperation for As2O3 and cinobufacin against angiogenesis in CAM, and the combination drug group is more effective in antiangiogensis in CAM.