CBS在小鼠卵泡发育过程中的表达模式及其功能初探

Expression Profile and Preliminary Functional Analysis of Cystathionine-β-synthase during Mice Follicular Development

目的:研究调节同型半胱氨酸水平的关键酶——胱硫醚β合成酶(cystathionine-β-synthase,CBS)在卵母细胞和卵泡发生过程中的动态表达模式及潜在的生物学作用。方法:以体外培养的卵泡以及去除卵泡中卵母细胞后培养的颗粒细胞为载体系统,利用半定量RT-PCR的方法,研究不同时期卵泡以及颗粒细胞中CBS的动态表达模式。结果:在各级卵泡的颗粒细胞中均有高水平的CBS表达,用完整卵泡进行的表达研究表明,各级卵泡中也都有CBS高水平的表达,同时期的颗粒细胞与完整卵泡相比,前者具有更高水平的CBS表达;而不同发育阶段的卵母细胞中均未见CBS表达。结论:CBS在卵泡和卵母细胞的生长、发育和成熟过程中起着重要的作用,其中CBS在卵泡和卵细胞中不同的表达模式提示CBS对卵母细胞发育的影响可能是通过卵泡细胞与卵母细胞的相互作用而实现的。

Objective： To understand the potential function ofcystathionine-β-synthase gene（CBS）, which is the key enzyme in the metabolism of homocysteine, during the oocyte and follicles development. Methods： By adoption of follicles in vitro growth and cultured granulose cells by oocytectomy, the expression of CBS in follicles and granulose cells were detected by semi-quantitative RT-PCR. Results： High expression level of CBS was detected in follicles and granulose cells through the whole stage of follicle development. In preantral follicles, the expression level of CBS was higher than that in antral follicles, and it was highest in graulose cells from preantral follicles. And there was a higher level of CBS expression in cumulus-oocyte complexes（COC）. At the same time, the level of CBS expression was higher in granulose cell clumps from preantral follicles and antral follicles than that in the follicles of the same development stage. But throughout the oocyte development, no CBS expression was detected in oocyte. Conclusion： The results show that CBS is involved in the whole stage of oocyte and follicle development. The specific CBS expression profile in follicle cells and oocyte indicates that it is probably by the interaction between oocyte and follicular cells for CBS to make its effect on oocyte development.