纳米铜/聚合物复合材料宫内节育器对猕猴宫腔液t-PA、PAF、PGE_2水平的影响

Impact of Nano-Cu/LDPE-IUD on the Level of t-PA、PAF、PGE_2 in the Uterine Fluid of Macaca mulatta

目的:探讨纳米铜/聚合物复合材料宫内节育器(IUD)对置器后子宫出血、疼痛的影响。方法:选择具规律月经周期且有生育史的育龄猕猴30只,分为空白对照组、裸铜组、生物材料组、低剂量纳米铜组(10μg/220mm2)、高剂量纳米铜组(20μg/220mm2),每组6只。采用酶联免疫吸附法(ELISA)检测各组置器后子宫宫腔冲洗液中t-PA、PAF、PGE2水平。结果:①与空白组相比,裸铜组宫腔冲洗液中t-PA及PGE2水平显著升高;②与裸铜组相比,纳米铜组t-PA及PGE2水平均显著降低;③除裸铜组PAF显著高于空白组外,其他各组间PAF水平无明显差异。结论:纳米铜IUD可减少对子宫t-PA和PGE2水平的影响,因而可能减轻置入IUD引起的子宫异常出血、疼痛副反应。

Objective： To explore the impact of Nano- Cu/LDPE-IUD on uterine bleeding and pain. Method： Thirty normal sexual mature Rhesus monkeys which had previously pregnant history, with normal menstruation were chosen as research objectives and divided into five model groups, including blank control group, biomaterial group, Cu-IUD group, low dose （10 μtg/220 mm^2） and high dose （20 μg/220 mm^2） Nano-Cu/LDPE-IUD group. The enzyme linked immunosorbent assay（ELISA） was performed to estimate the level of t-PA, PAF, PGE2 in the uterine fluid of IUD-inserted Macaca mulatta. Result： Compared with control group, the levels of both t-PA and PGE2 in the uterine fluid of Cu-IUD group significantly increased. Compared with Cu-IUD group, the levels of both t-PA and PGE2 in the uterine fluid of Nano-Cu/LDPE-IUD group significantly decreased（P〈0.05）. Except that the level of PAF in Cu-IUD group was significantly higher than that in control group（P〈0.05）, there was no significant difference among other groups. Conclusion： Nano-Cu/LDPE-IUD could lessen abnormal bleeding and pain caused by Cu-IUD through depress the rising level of t-PA and PGE2.