预激态补骨脂素对白血病原代细胞杀伤作用的研究

Cytotoxic effects of previously activated psoralens on leukemic cells

目的:体外观察预激态补骨脂素(PAP)对白血病原代细胞的杀伤作用。方法:6例临床确诊为白血病的血标本采用体外细胞培养方法,在白血病原代细胞培养板中加入PAP,使其浓度保持在0.05g/L。短期用药PAP浓度维持24h,7天时终止实验;长期用药PAP浓度维持5天,并分别在第7天、第10天、第14天时终止实验。以细胞总数、台盼蓝拒染率和台盼蓝拒染细胞抑制率等作为检测指标,以配对t检验进行组间比较。结果:短期用药和长期用药均显示PAP对白血病原代细胞具有显著杀伤作用。短期用药(≤7天),作用时间延长对PAP杀伤白血病细胞作用的影响不明确。长期用药结果显示台盼蓝拒染细胞抑制率从32.40%～69.50%升至94.10%～98.90%。难治或耐药与否对PAP杀伤白血病细胞作用无明显影响。结论:PAP对白血病原代细胞具有显著的杀伤作用,有可能成为一广谱抗白血病药物。

Objective：To investigate the cytotoxic effects of PAP on leukemic cells in vitro. Methods：Six blood samples were collected from six patients who had been diagnosed as acute leukemia clinically. PAP were added into the culture system after culture began. The concentration of PAP in the culture system were 0.05g/L. We kept this concentration of PAP in the culture system for 24 hours in short term PAP administration experiment where leukemic cells would be counted in the 7th day. This concentration of PAP in the culture system were kept for 5 days in long term PAP administration experiment which would be stoped in the 7th, 10th, and 14th day respectively. These two experiments were designed controlly to measure the total cell counts, trypan-blue-exclusion rate, and trypan-blue-exclusion inhibition rate. The statistical comparisons between two groups were conducted through matched t tests. Results： PAP was of significant cytotoxic effects on leukemic cells in both PAP administration experiments. The influence of the prolongation of PAP administration period in the cytotoxicity of PAP towards leukemic cells was indefinite in the circumstance of 7-day culture. Trypanblue-exclusion inhibition rates of leukemic cells were elevated significantly from 32.40% -69. 50% to 94. 10% - 98. 90% when culture period was prolonged from 7 days to 14 days, ie, the cytotoxicity of PAP towards leukemic cells was increased significantly. Regardless of refractory case or primarily resistant case, the cytotoxicity of PAP towards leukemic cells from these cases was not interfered. At the 14th day of culture the morphology of leukemic cells changed obviously in experiment group, but did not in control group. Some adherent cells grew in control group, but did not in experiment group. Conclusion：PAP is of significant cytotoxic effects on vari- ous leukemic cells and might become a broad spectrum anti-leukemic medicine.