肝细胞癌COX-2表达与新生血管生成关系的研究

The correlation between cyclooxygenase-2 expression and tumor angiogenesis in hepatocellular carcinoma

目的:探讨环氧合酶2(cyclooxygenase2,COX2)表达上调与人肝细胞癌(HCC)新生血管生成的关系。方法:2002～2003年本院病理诊断为HCC的80例手术标本,采用免疫组化方法,检测癌组织中COX2和血管内皮生长因子(Vascularendothelialgrowthfactor,VEGF)和CD34的表达情况,使用Weidner分析方法来计算CD34标记的肿瘤微血管密度(microvasculardensity,MVD)。结果:COX2和VEGF在HCC中表达率分别为75.00%(60/80)和62.50%(50/80)。VEGF在COX2强阳性组和中弱阳性组的免疫染色积分分别是5.98±1.16和3.30±0.22;VEGF在COX2强阳性组和中弱阳性组的表达率分别是71.43%(25/35)和28.00%(7/25),两组间差异非常显著(P<0.01),COX2与VEGF表达有明显相关性(P<0.01)。MVD在COX2强阳性组和中弱阳性组的评分分别为73.81±12.43和33.42±7.52,两组间差异非常显著(P<0.01),COX2与MVD有明显相关性(P<0.01)。结论:COX2高表达与HCC血管生成密切相关。

Objective：To explore the correlation between up-regulation of cyclooxygenase-2 （COX-2） expression and hepato- cellular carcinoma （HCC） angiogenesis. Methods：The expression of COX-2 and Vascular endothelial growth factor （VEGF） were examined in eighty cases matched sets of HCC specimens using immunohistochemistry. Weidner analysis system was used to assess the microvessel density（ MVD ） as a percentage of the endothelial area. Results： The expression rate of COX-2 and VEGF was 75.0% （ 60/ 80）, 62. 5% （50/80）, respectively. Immunohistochemical staining scores of VEGF was 5.98 ± 1.16 in strongly positive group of COX-2, and which was 3.30 ±0. 22 in moderately and weakly positive group of COX-2. The expression rates of VEGF was 71.43% （25/35） in strongly positive group of COX-2, and which was 28.00% （7/25） in moderately and weakly positive group of COX-2. There was significant difference in HCC angiogenesis in two groups （ P 〈 0. 01 ）, and COX-2 was significantly correlated with VEGF （ P 〈0. 01 ）. MVD was 73. 18 ± 12. 43 and 33.42 ±7. 52 in strongly positive group of COX-2 and moderately and weakly positive group of COX-2, respectively. There was significant difference in MVD（ P 〈 0. 01 ）, and COX-2 was significantly correlated with MVD（ P 〈 0.01 ）. Conclnsions：Up-regulation of COX-2 expression correlates with tumor angiogenesis in HCC.