急性白血病骨髓组织中低氧诱导因子-1α表达与血管生成关系的研究

The expression level of hypoxi-inducible factor-1α in bone marrow biopsies of acute leukemia and the relationship with angiogenesis

目的:了解低氧诱导因子(H IF)-1α在急性白血病骨髓组织中的表达水平,探讨其与骨髓内血管生成的关系。方法:应用改良的乙二醇甲基丙烯酸酯(GMA)塑料包埋法进行骨髓活检组织制片,采用EnV ison免疫组化二步法检测对照10例和20例初诊急性白血病骨髓组织中H IF-1α表达水平及微血管密度(MVD)。结果:20例急性白血病骨髓组织中H IF-1α蛋白低、中等、较高和高水平表达分别为1、5、6和8例;对照组10例中等、较高水平表达分别为7和3例,两组差异显著(P<0.05)。初诊急性白血病骨髓内MVD在40倍镜下为8.50±3.92,较对照组(4.71±1.53)明显增高(P<0.05)。急性白血病骨髓组织中H IF-1α蛋白表达水平与MVD具有相关性(r=0.578,P<0.05)。结论:H IF-1α在急性白血病骨髓组织中表达水平明显升高,可能对骨髓内异常新生血管生成起一定的调节作用。

Objective ： To explore the expression level of hypoxi-inducible factor-1 α and the relationship with angiogenesis in bone marrow biopsies of acute leukemias. Methods： Bone marrow biopsies from 10 controls and 20 acute leukemias were embedded in glycol-methacrylate （GMA） resin . EnVison twostep immunohistochemical staining method was used to show the expression level of HIF-1 α and microvessel density. Results： The low expression level of HIF-1α protein in acute leukemias was observed in 1 case, middle level in 5 cases, high level in 6 cases and very high level in 8 cases, while in controls, high level in 3 cases and middle level in 7 cases . There was statistical significance between two groups （ P 〈 0.05 ）. The hot spot MVD was 8.50 ± 3.92/（40 × HPF） in acute leukemias, 4.71 ± 1.53 / （40 × HPF） in controls. The difference between two groups was significant （P 〈 0.05 ）. Positive correlation was found between HIF-1α and MVD （r = 0. 568, P 〈 0.05 ）. Conclusion： The increased expression level of HIF-1 a in bone marrow biopsies of acute leukemias might play partial role in modulating angiogenesis.