骨髓间充质干细胞移植对心肌梗死后细胞凋亡的影响

Influence of mesenchymal stem cells transplantation on the cell apoptosis after myocardial infarction

目的:观察骨髓间充质干细胞移植对心肌梗死后心肌细胞凋亡的影响,探讨干细胞移植改善心功能机制。方法:实验于2004-09/2005-10在郧阳医学院附属十堰市太和医院生命科学研究所完成。①21只日本大耳白兔采用随机数字法分为正常对照组、心肌梗死组及干细胞移植组,每组7只。②心肌梗死模型制作3周后,心肌梗死组及干细胞移植组分别在梗死周边区域注射100μL细胞培养基或干细胞悬液,对照组不处理。③4周后心脏超声检测心功能、左室收缩末期内径、左室舒张末期内径等评价心室重塑。处死动物后经流式细胞仪检查梗死区域细胞凋亡情况。结果:①各组兔心脏超声检测结果:与心肌梗死组相比,干细胞移植治疗后,左心室射血分数[(56.27±1.73),(69.41±3.19)%,P<0.001]、左室收缩末期内径[(10.25±0.89),(8.13±0.79)mm,P<0.001]、左室舒张末期内径[(13.82±1.02),(11.24±1.21)mm,P<0.001]、室壁运动明显改善。②各组细胞凋亡检查结果:干细胞移植后,细胞凋亡显著降低[(14.00±0.31)%,(4.30±0.57)%,P<0.001]。结论:干细胞移植可抑制细胞凋亡,改善心功能,预防心室重塑,具有改善梗死后心脏功能的作用。

AIM： To observe the effect of mesenchymal stem cells （MSCs） on the myocardial cell apoptosis after myocardial infarction, and explore the improved cardiac function of stem cells. METHODS： The experiment was performed at the Institute of Life Science, Shiyan Taihe Hospital Affiliated to Yunyang Medical College from September 2004 to October 2005. ①A total of 21 Japanese flap-eared rabbits were divided into normal control group, myocardial infarction group and stem cell group with 7 rats in each group.②100 μL cell medium or stem cell suspension was implanted in the myocardial infarction group and stem cell group 3 weeks after the model of myocardial infarction were made. Control group did not receive any intervention, ③After 4 weeks, cardiac function, bore in left ventricular end-systolic stage and bore in left ventricular end-diastolic stage were measured with heart ultrasound to assess the ventricular remodeling. Apoptosis in infarcted region was examined with flow cytometer after killing animals. RESULTS： ① Result of cardiac ultrasound in rabbits of each group： Compared with myocardial infarction group, left ventricular ejection fraction [（56.27±1.73）, （69.41±3.19）%, P 〈 0.001], the bore of left ventricular end-systolic stage [（10.25±0.89）, （8.13±0.79）mm, P 〈 0.001], bore in left ventricular end-diastolic stage [（13.82±1.02）, （11.24±1.21） mm,P 〈 0.001] and ventricular wall motion were markedly improved after stem cell transplantation. ②Result of apoptosis examination： After stem cell transplantation, the apoptosis decreased obviously [（14.00±0.31）%, （4.30±0.57）%,P 〈 0.001]. CONCLUSION： Stem cell transplantation can inhibit cell apoptosis, improve cardiac function, provent ventricular remodeling and ameliorate cardiac function after infarction.