急性移植物抗宿主病病人血清IL-2、IL-8、IL-10及TNF-α水平变化的研究

Changes of IL-2, IL-8, IL-10 and TNF-α levels in Sera of Patients with Acute Graft-Versus-Host Disease

为了探讨细胞因子IL-2、TNF-α、IL-10、IL-8在异基因造血干细胞移植后aGVHD发病中的作用,观察33例Allo-HSCT患者aGVHD的发病情况。aGVHD的诊断主要依据临床表现,部分病例还依据皮肤、肠粘膜活检的病理学变化。移植前后定期采集患者血清,采用放射免疫法检测细胞因子IL-2、TNF-α、IL-10、IL-8的浓度的变化。结果发现,异基因造血干细胞移植后33例患者均获得造血功能重建,13例发生aGVHD,其中8例Ⅰ度aGVHD,5例Ⅱ-Ⅳ度aGVHD。IL-2、TNF-α水平在aGVHD阳性组明显高于aGVHD阴性组,而IL-10的水平在aGVHD阳性组明显低于aGVHD阴性组,IL-8水平的变化无统计学意义。结论细胞因子IL-2、TNF-α在临床aGVHD的发病中起重要的正向调节作用,定期检测患者血清的IL-2、TNF-α水平有助于预测aGVHD的发生。

The purpose of this study was to explore the roles of cytokines IL-2,TNF-α,IL-10 and IL-8 in the pathogenesis of acute graft-versus-host disease after allogeneic hematopoietic stem cell transplantation, The incidence of aGVHD was observed in 33 patients undergoing allogeneic hematopoietic stem cell transplantation. The aGVHD was clinically diagnosed. Sera from the 33 patients were taken before and after allogeneic hematopoietic stem cell transplantation. The IL- 2 ,TNF-α,IE-8, IL-10 levels in serum of 33 patients were measured serially by using radioimmuno-assay （RIA）, aGVHD occured in 13 patients including 8 patients with aGVHD Ⅰ and 5 patients with aGVHD Ⅱ - Ⅳ. The resuits showed that the circulating levels of IL-2 and TNF-α were markedly elevated during aGVHD and strongly correlated with the severity of aGVHD as compared with patients without aGVHD. However, the level of the IL-10 in patients with aGVHD was significantly lower than that in patients without aGVHD. The change of IL-8 level was not significant statistically. It is concluded that IL-2 and TNF-α may play important roles in the pathogenesis of aGVHD, and measurement of serum IL-2 and TNF-α levels after allo-HSCT can provide predictive indicator for acute GVHD.