全反式维甲酸对外周血干细胞移植患者骨髓基质细胞黏附分子表达及黏附力的影响

In Vitro Effect of All-Trans Retinoic Acid on Cell Adhesion Molecule Expression and Adhesion Capacity of Bone Marrow Stromal Cells in Patients Received Peripheral Blood Stem Cell Transplantation

本研究探讨全反式维甲酸(all-transretinoicacid,ATRA)能否促进外周血造血干细胞移植(peripheralbloodstemcelltransplantation,PBSCT)预处理致伤的骨髓基质细胞(bonemarrowstromalcells,BMSC)功能的恢复。用流式细胞术检测27例造血干细胞移植患者预处理前后BMSC表面细胞间黏附分子-1(intercellularadhesionmolecule-1,ICAM-1)和血管黏附分子-1(vascularadhesionmolecule-1,VCAM-1)表达,用放射免疫法测定BMSC培养上清液中可溶性ICAM-1(sICAM-1)水平以及BMSC对CD34+细胞的黏附率,并检测浓度分别为0.01、0.1、1μmol/L的ATRA作用后上述指标的动态变化。结果显示PBSCT预处理后,BMSC表面ICAM-1和VCAM-1的表达以及BMSC培养上清液中sICAM-1的表达水平降低,BMSC对CD34+细胞黏附率降低,而在ATRA作用后,BMSC表面ICAM-1表达增加,培养上清液中sICAM-1水平升高,BMSC对CD34+细胞的黏附率增加,但VCAM-1表达变化不明显。结论全反式维甲酸可部分修复受损的BMSC的黏附功能,有助于促进骨髓造血重建。

The aim of this study was to evaluate the effect of all-trans retinoic acid（ATRA） on cell adhesion molecule expression and adhesion capacity of bone marrow stromal cells （ BMSC ） in patients after conditioning treatment for peripheral blood stem cell transplantation （PBSCT）. BMSC of 27 patients before and after conditioning treatment for PBSCT were cultured in vitro. After treated with ATRA at 0.01,0. 1, or 1μmol/L, expression of intercellular adhesion molecule- 1 （ ICAM-1 ） protein and vascular adhesion molecule-1 （ VCAM-1 ） protein were detected by flow cytometry, and soluble ICAM-1 （sICAM-1） protein was determined by using radioimmunoassay, Then BMSC was co-cultured with CD34^＋ cells, and adhesion rate of BMSC to CD34^＋ cells was measured. The results showed that after pretreatment with conditioning regimen for PBSCT, the expressions of ICAM-1 and VCAM-1 proteins in BMSC and the expression level of slCAM-1 protein in supernatant of BMSC culture were down-regulated, and the adhesion rate of BMSC to CD34 ^＋ cells was decreased, after administration of ATRA, the expression of ICAM-1 protein in BMSC, sICAM-1 protein in culture medium and adhesion rate of BMSC to CD34^＋ cells all increased significantly, but expression of VCAM-1 protein changed no significantly. It is concluded that the ATRA can partly restore adhesion function of BMSC injured by pretreatment for PBSCT and contribute to hematopoietic reconstitution.