不同脱乙酰度对壳聚糖膜与角膜基质细胞相容性的影响

The Effect of the Degree of Deacetylation of Chitosan on the Biocompatibility of Chitosan Membrane with Corneal Stromal Cells

以分子量为30万,脱乙酰度分别为63.3%、73.7%、83%和97%的壳聚糖制备不同的壳聚糖膜,在不同脱乙酰度的壳聚糖膜上培养兔角膜基质细胞,通过观察角膜基质细胞在不同壳聚糖膜上的生长状态、贴附情况、生长曲线以及乳酸脱氢酶的活性,研究壳聚糖分子脱乙酰度对壳聚糖膜与角膜基质细胞生物相容性的影响。实验结果表明壳聚糖脱乙酰度越高,壳聚糖膜对细胞的损伤越小,越有利于细胞在膜上的生长和贴附,反之,低脱乙酰度的壳聚糖膜与角膜细胞的相容性较差。

Some different membranes were prepared by Chitosan with the degree of deacetylation （DD） of 63.7 %, 73.70%, 83 % and 97 % respectively. To study the biocompatibility of Chitosan membrane toward corneal seromal cells, the rabbit cells were cultured on the surface of different DD chitosan membranes. The morphological characteristics, the cell-adhesion, the cell proliferation and the activity of LDH in the medium were investigated. The results of experiment shows that the DD of Chitosan has very significant effect on the biocompatibility of Chitosan membrane toward corneal stromal cells. The more DD of Chitosan, the less injury was made to corneal stromal cells by the chitosan membrane, which is favor of the growing and adhesion of corneal stromal cells. The biocompatibility of the membrane made with low DD Chitosan with corneal stromal cells became worse.