生物工程活性角膜基质的研制及相容性研究

The Reconstruction of Bioengineer Active Corneal Stroma and Its Biocompatibility Study

研究生物工程活性角膜的生物相容性,为进一步临床应用提供理论基础。猪角膜基质脱细胞并去除免疫源性物质形成网状半透明生物材料,将培养的角膜基质细胞与生物材料复合构建生物工程活性角膜基质。对复合物进行倒置显微镜和扫描电镜检测细胞附着情况及材料的细胞相容性;将活性角膜基质移植入新西兰兔角膜囊袋内,细胞用B rdU标记检测在体内移植过程中的存活及转归,不同时间观察角膜的生物相容性及改建情况。结果显示脱细胞基质材料的细胞相容性较好,细胞种植后可存活、黏附并增殖;移植区细胞可有B rdU阳性着色,4周后角膜开始透明,8周后角膜改建基本完成。

This paper aims to explore the biocompatibility of bioengineer active corneal stroma （BACS）, as the biological carrier for cornea reconstruction, to provide the basis for future study on clinic application. The cells and immunogenic components of cornea stroma were removed through different extract methods. A complex of functional corneal stroma cells and acellular corneal stroma was used to reconstruct BACS. Their morphological characteristics and ultrastructures were observed with transmission electron microscope. The complex was grafted into interlamellar stromal pockets. Cells were labeled by BrdO to examine the survival and conversion after grafting. The cells could survive and proliferate in acellurar corneal stroma. All the nuclei of the corneal stromal cells showed positive labeling with BrdU in the BACS. After 4 weeks, BACS became transparent; after 8 weeks, the bioengineer active cornea stroma was fully reconstructed.