盐酸戊乙奎醚对氧化乐果中毒大鼠膈肌的作用

Effects of penehyclidine hydrochloride on the diaphragm of rats with omethoate poisoning

目的探讨氧化乐果染毒大鼠膈肌损害及盐酸戊乙奎醚对其保护作用。方法96只Wistar大鼠腹腔注射氧化乐果建立有机磷中毒模型,并给予阿托品及盐酸戊乙奎醚治疗,随机分为四组：盐水对照组、中毒对照组、阿托品+氯磷定治疗组、盐酸戊乙奎醚+氯磷定治疗组,测定各组大鼠染毒后2 h血液胆碱酯酶(AChF)和肌酸激酶(CK),观察染毒后不同时间膈肌组织病理学和超微结构的改变。结果盐酸戊乙奎醚治疗组大鼠各时段膈肌病理损害较阿托品治疗组明显为轻,病变恢复快。结论膈肌纤维坏死是有机磷农药中毒所致呼吸肌麻痹的可能原因之一,盐酸戊乙奎醚对氧化乐果中毒大鼠的膈肌有明确的保护作用,推测其有预防中间期肌无力的作用。

Objective To study the morphological changes of diaphragm of rats with omethoate poisoning and the protection of penehyclidine hydrochloride. Methods The experimental model of Wistar rats was made by eliac injection ofomethoate, 96 rats was divided randomly into four groups in average： the brinematched control group （Group NO） ; the omethoate intoxication matched control group （ Group PO ） ; atropine and pralidoxime chloride cure group （ Group AC ） ; penehyclidine hydrochloride and pralidoxime chloride cure group （Group PC）. The whole blood cholinesterase （ChE） and creatinekinase （CK） activtitise were measured 2 h after poisoning. To observe the morphological changes of diaphragmat different time from 1 to 7 days, Results All the poisoned rats showed that the diaphragmatic histologic damage of the penehyelidine hydrochloride and pralidoxime chloride cure group was slighter than that of atropine cure group. Conclusion A possible reasons of the respiratory muscle paralysis conduced by AOPP was the putrescence of diaphragm muscle fiber, and penehyclidine hydrochloride could definitely protect the diaphragm of rats with omethoate poisoning, and we could deduce that it prevented from intermediate myasthenia syndrome.